Engineered Metal-Binding Proteins: Purification to Protein Folding

• Published in: Science (American Association for the Advancement of Science) 1991-06, Vol.252 (5014), p.1796-1797
• Main Authors: Arnold, Frances H., Haymore, Barry L.
• Format: Article
• Language: English
• Subjects: 550200 - Biochemistry; 560300 - Chemicals Metabolism & Toxicology; AMINO ACIDS; AZOLES; BASIC BIOLOGICAL SCIENCES; Binding Sites; BIOCHEMICAL REACTION KINETICS; Biological and medical sciences; BIOTECHNOLOGY; CARBOXYLIC ACIDS; Carrier Proteins - chemical synthesis; Carrier Proteins - chemistry; Carrier Proteins - isolation & purification; Chelates; Chelation; CHEMICAL BONDS; Chemical engineering; Chemistry; COMPLEXES; Copper; COPPER COMPLEXES; Cytochrome c Group - chemistry; Cytochromes; Disulfides; folding; FRACTIONATION; Fundamental and applied biological sciences. Psychology; GENETIC ENGINEERING; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HISTIDINE; IMIDAZOLES; KINETICS; LIGANDS; MEMBRANE PROTEINS; METABOLISM; Metal ions; metal-binding protein; Metallurgy; Metals; Metals - metabolism; Methods. Procedures. Technologies; Models, Molecular; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; Perspective; Protein binding; Protein Conformation; PROTEIN ENGINEERING; Protein folding; PROTEINS; purification; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; REACTION KINETICS; RECEPTORS; reviews; SEPARATION PROCESSES; TRANSITION ELEMENT COMPLEXES; use
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1648261

Proteins can use metal ions to bind substrates, to maintain structure, to effect catalysis and for allosteric control and regulation. It is demonstrated that metal recognition can be engineered into proteins for applications such as protein purification.