Spatially resolved temperature measurements in electrophoresis capillaries by Raman thermometry

Local temperatures inside a 75-microns-i.d. capillary under electrophoresis conditions are measured noninvasively with a Raman microprobe. The method is based on the temperature dependence of the water O-H stretch equilibrium between weakly bent and strongly bent hydrogen bonded species. With calibr...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1993-02, Vol.65 (3), p.293-298
Main Authors: Davis, Kevin L, Liu, Kei Lee K, Lanan, Maureen, Morris, Michael D
Format: Article
Language:English
Subjects:
400102 - Chemical & Spectral Procedures
400400 - Electrochemistry
Analytical chemistry
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
Chemistry
Chromatographic methods and physical methods associated with chromatography
DATA
ELECTROPHORESIS
Electrophoresis - instrumentation
ENERGY TRANSFER
Exact sciences and technology
HEAT TRANSFER
HYDROGEN COMPOUNDS
INFORMATION
INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
LASER SPECTROSCOPY
MATHEMATICAL MODELS
NUMERICAL DATA
ORGANS
Other chromatographic methods
OXYGEN COMPOUNDS
RAMAN SPECTROSCOPY
SPECTROSCOPY
Spectrum Analysis, Raman - instrumentation
Temperature
TEMPERATURE MEASUREMENT
THEORETICAL DATA
WATER
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Description
Summary:Local temperatures inside a 75-microns-i.d. capillary under electrophoresis conditions are measured noninvasively with a Raman microprobe. The method is based on the temperature dependence of the water O-H stretch equilibrium between weakly bent and strongly bent hydrogen bonded species. With calibration against a known temperature standard, this technique is shown to be capable of obtaining spatially resolved intracapillary temperature measurements with an accuracy of +/- 1.0 degree C and a precision of +/- 0.1 degree C. Intracapillary temperatures ranging from 25 to 70 degrees C are observed over the range of buffer compositions and electric fields used. Differences between local temperatures and average capillary temperatures are observed at all operating conditions. The difficulty of an accurate theoretical description of heat dissipation under CE conditions is discussed.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00051a018