Haplotypes of the human immunoglobulin lambda IGLV7SI and IGLVISI genes defined by restriction-site polymorphism and insertion/deletion of a 6-kb DNA fragment

Haplotypes were defined in the human immunoglobulin lambda locus by using three probes--V lambda VII, V lambda A, and V lambda I--hybridized to BamHI, KpnI, EcoRI, and HindIII digests. Four KpnI alleles were described. Two of them, 13 kb and 16 kb, detected with both the V lambda VII and V lambda A...

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Bibliographic Details
Published in:American journal of human genetics 1993-08, Vol.53 (2), p.518-525
Main Authors: Chuchana, P, Frippiat, J-P, Blancher, A, Lefranc, G, Lefranc, M-P
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
BIOLOGICAL VARIABILITY
deletion
DNA
DNA HYBRIDIZATION
fragments
GENES
GENETIC VARIABILITY
GLOBULINS
haplotypes
HUMAN POPULATIONS
HYBRIDIZATION
IMMUNOGLOBULINS
insertion
lambda chain
light chains
man
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
POPULATIONS
PROTEINS 550400 > Genetics
restriction fragment length polymorphism
RFLPS
variable region
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Summary:Haplotypes were defined in the human immunoglobulin lambda locus by using three probes--V lambda VII, V lambda A, and V lambda I--hybridized to BamHI, KpnI, EcoRI, and HindIII digests. Four KpnI alleles were described. Two of them, 13 kb and 16 kb, detected with both the V lambda VII and V lambda A probes, were correlated with 4.6-kb and 10.5-kb KpnI fragments, respectively, which hybridize to the V lambda I probe. The two others (17 kb and 24 kb) were detected with the three probes V lambda VII, V lambda A, and V lambda I. Moreover, we show that two of those haplotypes may reflect an insertion of 6 kb between V lambda A and V lambda 1S1. Familial studies were performed to demonstrate the Mendelian inheritance. Our results demonstrate the absence of association between the C lambda alleles and V lambda haplotypes.
ISSN:0002-9297
1537-6605