Diffusion and Binding of Monoclonal Antibody TNT-1 in Multicellular Tumor Spheroids

Tumor spheroids of HT-29 human colon adenocarcinoma and A375 melanoma were established to investigate the uptake and clearance kinetics of TNT-1, a monoclonal antibody that targets ne-crotic cells of tumors. Our data reveal that there was rapid uptake of TNT-1 and its F(ab‘)2 fragment in both sphero...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1991-02, Vol.83 (3), p.200-205
Main Authors: Chen, Feng-Ming, Hansen, Eric B., Taylor, Clive R., Epstein, Alan L.
Format: Article
Language:English
Subjects:
550604 - Medicine- Unsealed Radionuclides in Therapy- (1980-)
Adenocarcinoma - metabolism
ANIMAL CELLS
ANIMALS
ANTIBODIES
Antibodies, Monoclonal - metabolism
Antineoplastic agents
Autoradiography
Binding Sites, Antibody
Biological and medical sciences
BODY
CARCINOMAS
Cell Division - physiology
Colonic Neoplasms - metabolism
Diffusion
DIGESTIVE SYSTEM
DISEASES
GASTROINTESTINAL TRACT
General aspects
Histones - immunology
Humans
IMMUNOLOGY
IMMUNOTHERAPY
INTESTINES
LARGE INTESTINE
MAMMALS
MAN
Medical sciences
MEDICINE
Melanoma - metabolism
MELANOMAS
METABOLISM
METASTASES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEAR MEDICINE
ORGANS
Pharmacology. Drug treatments
PRIMATES
RADIOIMMUNOLOGY
RADIOIMMUNOTHERAPY
RADIOLOGY
RADIOLOGY AND NUCLEAR MEDICINE
RADIOTHERAPY
THERAPY
Thymidine - metabolism
Tritium
TUMOR CELLS
Tumor Cells, Cultured - cytology
Tumor Cells, Cultured - metabolism
VERTEBRATES
Citations: Items that cite this one
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Description
Summary:Tumor spheroids of HT-29 human colon adenocarcinoma and A375 melanoma were established to investigate the uptake and clearance kinetics of TNT-1, a monoclonal antibody that targets ne-crotic cells of tumors. Our data reveal that there was rapid uptake of TNT-1 and its F(ab‘)2 fragment in both spheroid models, whereas an antibody of irrelevant specificity, Lym-1, and its F(ab’)2 fragment bound poorly to the spheroids. Unlike previously reported monoclonal antibodies to tumor cell-surface antigens, TNT-1 showed 1) a linear uptake that increased over time without saturation in tumor spheroids and 2) an unexpected uptake by a sub-population of cells in the viable outer rim of the spheroids. These preclinical studies provide important information concerning the therapeutic potential of TNT monoclonal antibodies for the treatment of cancer and micro-metastases. [J Natl Cancer Inst 83:200–204, 1991]
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/83.3.200