Detoxification of L-canavanine by the tobacco budworm, Heliothis virescens [Noctuidae]

The detoxification of L-canavanine and the ability of this natural product to induce a protein able to detoxify it were investigated in the tobacco budworm, Heliothis virescens. The available evidence indicates that this detoxification mechanism is part of larval constitutive metabolism and not indu...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1990-11, Vol.38 (11), p.2061-2065
Main Authors: Berge, Milan A, Rosenthal, Gerald A
Format: Article
Language:English
Subjects:
550501 - Metabolism- Tracer Techniques
AMINO ACIDS
ANIMALS
ARTHROPODS
BASIC BIOLOGICAL SCIENCES
Biochemistry. Physiology. Immunology
Biological and medical sciences
BIOLOGICAL PATHWAYS
CANAVANINA
CANAVANINE
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DEGRADACION
DEGRADATION
DESINTOXICACION
DETOXIFICATION
Fundamental and applied biological sciences. Psychology
GUANIDINA
GUANIDINE
GUANIDINES
HELIOTHIS VIRESCENS
Insecta
INSECTICIDES
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LARVAE
LARVAS
LARVE
LEPIDOPTERA
METABOLISM
MOTHS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PESTICIDES
Physiology. Development
TOLERANCE
TRACER TECHNIQUES
UREA
UREE
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Summary:The detoxification of L-canavanine and the ability of this natural product to induce a protein able to detoxify it were investigated in the tobacco budworm, Heliothis virescens. The available evidence indicates that this detoxification mechanism is part of larval constitutive metabolism and not induced in response to canavanine. H. virescens larvae, administered 5 mg/g L-canavanine supplemented with 37.7 kBq L-(guanidinooxy-{sup 14}C)canavanine, were sacrificied 0, 1, 2, 4, 6, and 12 h postinjection. The principle products of L-canavanine degradation were ({sup 14}C)guanidine and ({sup 14}C)urea. Homoserine formation was confirmed by automated amino acid analysis. This study demonstrates that the principal detoxification pathway for L-canavanine in H. virescens larvae is by reductive cleavage to guanidine and L-homoserine.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00101a013