Stereospecific Inositol 1,4,5-[32P]Trisphosphate Binding to Isolated Rat Liver Nuclei: Evidence for Inositol Trisphosphate Receptor-Mediated Calcium Release from the Nucleus

• Published in: Proceedings of the National Academy of Sciences - PNAS 1990-12, Vol.87 (23), p.9270-9274
• Main Authors: Malviya, A. N., Rogue, P., Vincendon, G.
• Format: Article
• Language: English
• Subjects: 550201 - Biochemistry- Tracer Techniques; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METAL COMPOUNDS; ANIMALS; ATP; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOCHEMICAL REACTION KINETICS; Biological and medical sciences; BIOLOGICAL LOCALIZATION; BODY; Calcium; Calcium - metabolism; CALCIUM 45; Calcium Channels; CALCIUM COMPOUNDS; CALCIUM ISOTOPES; CELL CONSTITUENTS; CELL MEMBRANES; CELL NUCLEI; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Cell physiology; Chemical suspensions; Cytochromes; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ESTERS; EVEN-ODD NUCLEI; Fundamental and applied biological sciences. Psychology; GLANDS; Heparin; Homogenization; Inositol 1; Inositol 1,4,5-Trisphosphate - metabolism; Inositol 1,4,5-Trisphosphate - pharmacology; Inositol 1,4,5-Trisphosphate Receptors; Inositols; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; LIPIDS; LIVER; Liver - metabolism; MAMMALS; MEMBRANE PROTEINS; MEMBRANES; MICROSOMES; Microsomes, Liver - metabolism; Molecular and cellular biology; NADPH-Ferrihemoprotein Reductase - metabolism; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANOIDS; ORGANS; PHOSPHOLIPIDS; PHOSPHORUS 32; PHOSPHORUS ISOTOPES; Phosphorus Radioisotopes; PROTEINS; RADIOISOTOPES; RATS; REACTION KINETICS; RECEPTORS; Receptors, Cell Surface - metabolism; Receptors, Cell Surface - physiology; Receptors, Cytoplasmic and Nuclear; RIBOSOMES; RODENTS; Second Messenger Systems; SECRETION; Signal Transduction; TRACER TECHNIQUES; VERTEBRATES
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.87.23.9270

It is well known that inositol 1,4,5-trisphosphate binding and release of calcium are mediated by the same protein. Several reports have indicated the location of the inositol 1,4,5-trisphosphate receptor in organelles other than endoplasmic reticulum. Immunocytochemical studies on the subcellular localization of 1,4,5-trisphosphate receptor in the Purkinje cells from two laboratories have given contradictory results regarding the nuclear location of this receptor. In this paper, a high-affinity inositol 1,4,5-[32P]trisphosphate binding site (Kd= 0.11 nM) on nuclei isolated from rat liver and devoid of any microsomal, mitochondrial, or plasma membrane constituents is documented. Furthermore, we present data demonstrating that inositol 1,4,5-trisphosphate is capable of releasing45Ca2+from the intact isolated liver nuclei. A rapid and transient release of calcium that was taken up by nuclei in the presence of ATP is observed. The role of inositol 1,4,5-trisphosphate in the coupling between cytoplasmic second messengers and nuclear events activated during signal transduction is postulated.