Effects of Recombinant Human Granulocyte Colony-Stimulating Factor on the Hematologic Recovery and Survival of Irradiated Mice

• Published in: Blood 1990-08, Vol.76 (3), p.445-449
• Main Authors: Tanikawa, Shu, Nose, Masako, Aoki, Yoshiro, Tsuneoka, Kazuko, Shikita, Mikio, Nara, Nobuo
• Format: Article
• Language: English
• Subjects: 560152 - Radiation Effects on Animals- Animals; ANIMAL TISSUES; ANIMALS; Biological and medical sciences; BIOLOGICAL RECOVERY; Blood Cell Count - drug effects; Blood Cell Count - radiation effects; BLOOD COUNT; BODY; BONE MARROW; Bone Marrow - drug effects; Bone Marrow Cells; COLONY FORMATION; Colony-Stimulating Factors - administration & dosage; Colony-Stimulating Factors - pharmacology; Colony-Stimulating Factors - therapeutic use; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; DOSE-RESPONSE RELATIONSHIPS; ELECTROMAGNETIC RADIATION; Femur - drug effects; Femur - radiation effects; Granulocyte Colony-Stimulating Factor; GROWTH FACTORS; HEMATOPOIETIC SYSTEM; Hematopoietic System - cytology; Hematopoietic System - drug effects; Immunomodulators; INJECTION; Injections, Intraperitoneal; INTAKE; INTRAPERITONEAL INJECTION; IONIZING RADIATIONS; Male; MAMMALS; Medical sciences; MICE; MITOGENS; ORGANIC COMPOUNDS; ORGANS; Pharmacology. Drug treatments; PROTEINS; Radiation Injuries, Experimental - drug therapy; Radiation Injuries, Experimental - mortality; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RADIATIONS; RADIOSENSITIVITY EFFECTS; Recombinant Proteins - administration & dosage; Recombinant Proteins - pharmacology; Recombinant Proteins - therapeutic use; RECOVERY; RODENTS; SPLEEN; SURVIVAL CURVES; Time Factors; TISSUES; VERTEBRATES; X RADIATION
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V76.3.445.445

We studied the effects of intraperitoneal injections of recombinant human granulocyte colony-stimulating factor (rhG-CSF) according to various administration schedules on the recovery of spleen colony-forming units (CFU-S) and peripheral blood counts, and on the survival of irradiated mice. The sooner and more frequently the mice were injected with rhG-CSF after irradiation, the more enhanced the recovery of CFU-S in bone marrow was obtained on day 7. Twice-daily injections of rhG-CSF from day 0 to day 2 significantly enhanced the recovery of platelets and hematocrit, but two injections of rhG-CSF on only day 0 did not. Twice-daily injections of rhG-CSF from day 0 to day 6 enhanced the recovery of platelets more effectively than twice-daily injections of rhG-CSF from day 1 to day 7, and increased the survival of irradiated mice more effectively than any other examined administration schedules. Twice-daily injections of rhG-CSF from day 0 to day 6 were significantly effective in enhancing the survival of mice irradiated with 8.5-, 9.0-, and 9.5-Gy x-rays, although not effective after irradiation of 10.5-Gy x-rays.