A hamster-human subchromosomal hybrid cell panel for chromosome 2

We have constructed hamster-human hybrid cell lines containing fragments of human chromosome 2 as their only source of human DNA. Microcell-mediated chromosome transfer was used to transfer human chromosome 2 from a monochromosomal mouse-human hybrid line to a radiation-sensitive hamster mutant (XR-...

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Bibliographic Details
Published in:Somatic cell and molecular genetics 1993, Vol.19 (1), p.39-49
Main Authors: JEGGO, P. A, HAFEZPARAST, M, THOMPSON, A. F, KAUR, G. P, SANDHU, A. K, ATHWAL, R. S
Format: Article
Language:English
Subjects:
AMINES
Animals
AROMATICS
AZAARENES
Base Sequence
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
chromosome 2
Chromosome Mapping - methods
CHROMOSOMES
Chromosomes, Human, Pair 2
Classical genetics, quantitative genetics, hybrids
Cricetinae
DNA Fingerprinting
DNA, Single-Stranded
Ecogpt gene
ENZYMES
ESTERS
Fundamental and applied biological sciences. Psychology
GENES
GENETIC MAPPING
Genetics of eukaryotes. Biological and molecular evolution
GLYCOSYL TRANSFERASES
GUANINE
hamsters
HETEROCYCLIC COMPOUNDS
Human
HUMAN CHROMOSOME 2
HUMAN CHROMOSOMES
Humans
Hybrid Cells - radiation effects
HYDROXY COMPOUNDS
In Situ Hybridization
man
MAPPING
Molecular Sequence Data
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PENTOSYL TRANSFERASES
PHOSPHORIC ACID ESTERS
Polymerase Chain Reaction
PROTEINS
PURINES
Repetitive Sequences, Nucleic Acid
RIBOSIDES
TRANSFERASES 550400 > Genetics
XANTHINES
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Description
Summary:We have constructed hamster-human hybrid cell lines containing fragments of human chromosome 2 as their only source of human DNA. Microcell-mediated chromosome transfer was used to transfer human chromosome 2 from a monochromosomal mouse-human hybrid line to a radiation-sensitive hamster mutant (XR-V15B) defective in double-strand break rejoining. The human chromosome 2 carried the Ecogpt gene and hybrids were selected using this marker. The transferred human chromosome was frequently broken, and the resulting microcell hybrids contained different sized segments of the q arm of chromosome 2. Two microcell hybrids were irradiated and fused to XR-V15B to generate additional hybrids bearing reduced amounts of human DNA. All hybrids were analyzed by PCR using primers specific for 27 human genes located on chromosome 2. From these data we have localized the integrated gpt gene on the human chromosome 2 to the region q36-37 and present a gene order for chromosome 2 markers.
ISSN:0740-7750
1572-9931
DOI:10.1007/BF01233953