Erythropoietin Retards DNA Breakdown and Prevents Programmed Death in Erythroid Progenitor Cells

The mechanism by which erythropoietin controls mammalian erythrocyte production is unknown. Labeling experiments in vitro with [$^3$H]thymidine demonstrated DNA cleavage in erythroid progenitor cells that was accompanied by DNA repair and synthesis. Erythropoietin reduced DNA cleavage by a factor of...

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Published in:Science (American Association for the Advancement of Science) 1990-04, Vol.248 (4953), p.378-381
Main Authors: Koury, Mark J., Bondurant, Maurice C.
Format: Article
Language:English
Subjects:
3T3 cells
Analytical, structural and metabolic biochemistry
ANIMALS
AZINES
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
BIOLOGICAL FUNCTIONS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
Cell culture techniques
Cell death
CELL DIFFERENTIATION
Cellular biology
CLEAVAGE
CRYSTAL STRUCTURE
Cultured cells
DNA
DNA - biosynthesis
DNA - drug effects
DNA - metabolism
DNA cleavage
DNA REPAIR
DNA REPLICATION
Epics
Erythrocyte Aging - drug effects
Erythroid cells
Erythroid Precursor Cells - cytology
Erythroid Precursor Cells - drug effects
Erythroid Precursor Cells - metabolism
Erythroid progenitor cells
Erythropoiesis
ERYTHROPOIETIN
Erythropoietin - pharmacology
Friend murine leukemia virus
FUNCTIONS
Fundamental and applied biological sciences. Psychology
Gels
Glycoproteins
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
Leukemia, Erythroblastic, Acute
MAMMALS
MICE
MICROSTRUCTURE
MITOGENS
Molecular Weight
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
Physiological aspects
PROTEINS
PYRIMIDINES
RECOVERY
REPAIR
RIBOSIDES
RODENTS
Stem cells
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES 550201 > Biochemistry-- Tracer Techniques
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cited_by cdi_FETCH-LOGICAL-c762t-b9bb2eef43a0e1efbcb30832e9b3fb6fc7b474034c546eeab9acfd16997380f03
cites cdi_FETCH-LOGICAL-c762t-b9bb2eef43a0e1efbcb30832e9b3fb6fc7b474034c546eeab9acfd16997380f03
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container_issue 4953
container_start_page 378
container_title Science (American Association for the Advancement of Science)
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creator Koury, Mark J.
Bondurant, Maurice C.
description The mechanism by which erythropoietin controls mammalian erythrocyte production is unknown. Labeling experiments in vitro with [$^3$H]thymidine demonstrated DNA cleavage in erythroid progenitor cells that was accompanied by DNA repair and synthesis. Erythropoietin reduced DNA cleavage by a factor of 2.6. In the absence of erythropoietin, erythroid progenitor cells accumulated DNA cleavage fragments characteristic of those found in programmed cell death (apoptosis) by 2 to 4 hours and began dying by 16 hours. In the presence of erythropoietin, the progenitor cells survived and differentiated into reticulocytes. Thus, apoptosis is a major component of normal erythropoiesis, and erythropoietin controls erythrocyte production by retarding DNA breakdown and preventing apoptosis in erythroid progenitor cells.
doi_str_mv 10.1126/science.2326648
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Labeling experiments in vitro with [$^3$H]thymidine demonstrated DNA cleavage in erythroid progenitor cells that was accompanied by DNA repair and synthesis. Erythropoietin reduced DNA cleavage by a factor of 2.6. In the absence of erythropoietin, erythroid progenitor cells accumulated DNA cleavage fragments characteristic of those found in programmed cell death (apoptosis) by 2 to 4 hours and began dying by 16 hours. In the presence of erythropoietin, the progenitor cells survived and differentiated into reticulocytes. 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Labeling experiments in vitro with [$^3$H]thymidine demonstrated DNA cleavage in erythroid progenitor cells that was accompanied by DNA repair and synthesis. Erythropoietin reduced DNA cleavage by a factor of 2.6. In the absence of erythropoietin, erythroid progenitor cells accumulated DNA cleavage fragments characteristic of those found in programmed cell death (apoptosis) by 2 to 4 hours and began dying by 16 hours. In the presence of erythropoietin, the progenitor cells survived and differentiated into reticulocytes. 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Bondurant, Maurice C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c762t-b9bb2eef43a0e1efbcb30832e9b3fb6fc7b474034c546eeab9acfd16997380f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>3T3 cells</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>ANIMALS</topic><topic>AZINES</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL FUNCTIONS</topic><topic>BIOLOGICAL RECOVERY</topic><topic>BIOLOGICAL REPAIR</topic><topic>Cell culture techniques</topic><topic>Cell death</topic><topic>CELL DIFFERENTIATION</topic><topic>Cellular biology</topic><topic>CLEAVAGE</topic><topic>CRYSTAL STRUCTURE</topic><topic>Cultured cells</topic><topic>DNA</topic><topic>DNA - biosynthesis</topic><topic>DNA - drug effects</topic><topic>DNA - metabolism</topic><topic>DNA cleavage</topic><topic>DNA REPAIR</topic><topic>DNA REPLICATION</topic><topic>Epics</topic><topic>Erythrocyte Aging - drug effects</topic><topic>Erythroid cells</topic><topic>Erythroid Precursor Cells - cytology</topic><topic>Erythroid Precursor Cells - drug effects</topic><topic>Erythroid Precursor Cells - metabolism</topic><topic>Erythroid progenitor cells</topic><topic>Erythropoiesis</topic><topic>ERYTHROPOIETIN</topic><topic>Erythropoietin - pharmacology</topic><topic>Friend murine leukemia virus</topic><topic>FUNCTIONS</topic><topic>Fundamental and applied biological sciences. 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Labeling experiments in vitro with [$^3$H]thymidine demonstrated DNA cleavage in erythroid progenitor cells that was accompanied by DNA repair and synthesis. Erythropoietin reduced DNA cleavage by a factor of 2.6. In the absence of erythropoietin, erythroid progenitor cells accumulated DNA cleavage fragments characteristic of those found in programmed cell death (apoptosis) by 2 to 4 hours and began dying by 16 hours. In the presence of erythropoietin, the progenitor cells survived and differentiated into reticulocytes. Thus, apoptosis is a major component of normal erythropoiesis, and erythropoietin controls erythrocyte production by retarding DNA breakdown and preventing apoptosis in erythroid progenitor cells.</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>2326648</pmid><doi>10.1126/science.2326648</doi><tpages>4</tpages></addata></record>
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identifier ISSN: 0036-8075
ispartof Science (American Association for the Advancement of Science), 1990-04, Vol.248 (4953), p.378-381
issn 0036-8075
1095-9203
language eng
recordid cdi_osti_scitechconnect_6650444
source Science Online; JSTOR Archival Journals; ProQuest Social Science Premium Collection; Education Collection
subjects 3T3 cells
Analytical, structural and metabolic biochemistry
ANIMALS
AZINES
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
BIOLOGICAL FUNCTIONS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
Cell culture techniques
Cell death
CELL DIFFERENTIATION
Cellular biology
CLEAVAGE
CRYSTAL STRUCTURE
Cultured cells
DNA
DNA - biosynthesis
DNA - drug effects
DNA - metabolism
DNA cleavage
DNA REPAIR
DNA REPLICATION
Epics
Erythrocyte Aging - drug effects
Erythroid cells
Erythroid Precursor Cells - cytology
Erythroid Precursor Cells - drug effects
Erythroid Precursor Cells - metabolism
Erythroid progenitor cells
Erythropoiesis
ERYTHROPOIETIN
Erythropoietin - pharmacology
Friend murine leukemia virus
FUNCTIONS
Fundamental and applied biological sciences. Psychology
Gels
Glycoproteins
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
Leukemia, Erythroblastic, Acute
MAMMALS
MICE
MICROSTRUCTURE
MITOGENS
Molecular Weight
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
Physiological aspects
PROTEINS
PYRIMIDINES
RECOVERY
REPAIR
RIBOSIDES
RODENTS
Stem cells
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques
title Erythropoietin Retards DNA Breakdown and Prevents Programmed Death in Erythroid Progenitor Cells
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