Effect of media supplementation on ethanol production by simultaneous saccharification and fermentation process

In this study, fermentation tests of different initial glucose concentrations, ranging from 100 to 200 g/L, were conducted to identify the ethanol tolerance of Kluyveromyces marxianus (EMS-26 strain) at 42[degrees]C. Lipid and nutrient additions to the fermentation medium were made in order to impro...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1994-03, Vol.45 (1), p.283-294
Main Authors: Ballesteros, I, Oliva, J.M, Carrasco, J.C, Ballesteros, M
Format: Article
Language:English
Subjects:
09 BIOMASS FUELS
ACIDE GRAS INSATURE
ACIDOS GRASOS INSATURADOS
ALCOHOLS
ALDEHYDES
ALMIDON
AMIDON
BACTERIA
BIOCONVERSION
Biological and medical sciences
BIOLOGICAL PATHWAYS
Biology of microorganisms of confirmed or potential industrial interest
BIOSYNTHESIS
Biotechnology
CARBOHYDRATES
CELLULASE
CELLULOLYSE
CELLULOLYSIS
CELLULOSE
CELLULOSES
CELULASA
CELULOLISIS
CELULOSA
CULTURE MEDIA
ESTEROLES
ETANOL
ETHANOL
FERMENTACION
FERMENTATION
Fundamental and applied biological sciences. Psychology
GLUCOSE
HEXOSES
HIDROLISIS
HYDROLYSE
HYDROLYSIS
HYDROXY COMPOUNDS
KLUYVEROMYCES MARXIANUS
MEDIO DE CULTIVO
MICROORGANISMS
MILIEU DE CULTURE
Mission oriented research
MONOSACCHARIDES
ORGANIC COMPOUNDS
Physiology and metabolism
PRODUCCION
PRODUCTION
SACCHARIDES
STARCH
STEROL
STEROLS
SYNTHESIS 090900 > Biomass Fuels-- Processing-- (1990-)
TOLERANCE
UNSATURATED FATTY ACIDS
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Summary:In this study, fermentation tests of different initial glucose concentrations, ranging from 100 to 200 g/L, were conducted to identify the ethanol tolerance of Kluyveromyces marxianus (EMS-26 strain) at 42[degrees]C. Lipid and nutrient additions to the fermentation medium were made in order to improve the ethanol production at 42[degrees]C. The results obtained showed that the addition of unsaturated fatty acids and sterols to the SSF media reduced hydrolysis yields and ethanol production as compared to tests carried out on controls without lipid supplementation. These results seem to indicate that cellulases performed better when no lipid supplementation was used. The decrease in the saccharification in the presence of 0.5% Tween 80 (10% decrease in the filter paper activity) seems to be closely related to the denaturation of the cellulase complex rather than lipids interference with cellulase binding. 22 refs., 4 figs., 2 tabs.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02941806