Cryobiological preservation of Drosophila embryos

The inability to cryobiologically preserve the fruit fly Drosophila melanogaster has required that fly stocks be maintained by frequent transfer of adults. This method is costly in terms of time and can lead to loss of stocks. Traditional slow freezing methods do not succeed because the embryos are...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1992-12, Vol.258 (5090), p.1932-1935
Main Authors: Mazur, P. (University of Tennessee, Oak Ridge, TN), Cole, K.W, Hall, J.W, Schreuders, P.D, Mahowald, A.P
Format: Article
Language:English
Subjects:
550400 > Genetics
ANIMALS
ARTHROPODS
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
BIOLOGICAL EFFECTS
BIOLOGY
Climate
CONGELACION
CONGELATION
CRYOBIOLOGY
CRYOGENICS
Cryopreservation - methods
Developmental Stages
DIPTERA
DROSOPHILA
DROSOPHILA MELANOGASTER
Drosophila melanogaster - cytology
Drosophila melanogaster - embryology
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - physiology
Embryonic and Fetal Development
FLIES
FREEZING
FRUIT FLIES
Fundamental and applied biological sciences. Psychology
Injuries
Insecta
INSECTS
Invertebrates
INVERTEBRATES 560200 > Thermal Effects
Life cycle. Embryology. Development
Physiology. Development
PRESERVACION
PRESERVATION
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
Reproduction
Time Factors
Water
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The inability to cryobiologically preserve the fruit fly Drosophila melanogaster has required that fly stocks be maintained by frequent transfer of adults. This method is costly in terms of time and can lead to loss of stocks. Traditional slow freezing methods do not succeed because the embryos are highly sensitive to chilling. With the procedures described here, 68 percent of precisely staged 15-hour Oregon R (wild-type) embryos hatch after vitrification at -205 degrees C, and 40 percent of the resulting larvae develop into normal adult flies. These embryos are among the most complex organisms successfully preserved by cryobiology
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1470915