Formation of α-Deoxyadenosine in Polydeoxynucleotides Exposed to Ionizing Radiation under Anoxic Conditions

When poly(dA), poly(dA-dT), and salmon testis DNA were γ-irradiated under nitrogen, the major deoxyadenosine damage product (excluding liberated adenine) was identified as the α-anomer of deoxyadenosine. The yields of α-deoxyadenosine from poly(dA), poly(dA-dT), and salmon testis DNA irradiated with...

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Bibliographic Details
Published in:Radiation research 1990-03, Vol.121 (3), p.328-337
Main Authors: Lesiak, Krystyna B., Wheeler, Kenneth T.
Format: Article
Language:English
Subjects:
ADENOSINE
ALDEHYDES
ANADROMOUS FISHES
ANIMALS
ANOXIA
AQUATIC ORGANISMS
Atoms
Biological and medical sciences
BIOSYNTHESIS
CARBOHYDRATES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
Chromatography, High Pressure Liquid
Deoxyadenosines - radiation effects
DEOXYRIBOSE
DNA
DNA - radiation effects
DNA damage
ELECTROMAGNETIC RADIATION
FISHES
Fundamental and applied biological sciences. Psychology
GAMMA RADIATION
Gamma Rays
IONIZING RADIATIONS
Isomers
MAGNETIC RESONANCE
Magnetic Resonance Spectroscopy
MOLECULAR STRUCTURE
MONOSACCHARIDES
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
Oxygen
PENTOSES
Poly A - radiation effects
Poly dA-dT - radiation effects
Polydeoxyribonucleotides - radiation effects
Polymers
Purines
RADIATION CHEMISTRY
Radiation damage
RADIATION EFFECTS
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RADIATIONS
RESONANCE
RIBOSIDES
SACCHARIDES
SALMON
Space life sciences
SYNTHESIS
Testes
Tissues, organs and organisms biophysics
VERTEBRATES 560120 > Radiation Effects on Biochemicals, Cells, & Tissue Culture
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Summary:When poly(dA), poly(dA-dT), and salmon testis DNA were γ-irradiated under nitrogen, the major deoxyadenosine damage product (excluding liberated adenine) was identified as the α-anomer of deoxyadenosine. The yields of α-deoxyadenosine from poly(dA), poly(dA-dT), and salmon testis DNA irradiated with a dose of 500 Gy under anoxic conditions were 1.5, 1.3, and 1.3%, respectively. No α-deoxyadenosine was detected after irradiation under oxic conditions. The presence of nucleotides with the α-configuration at the anomeric carbon atom in the DNA chain may have a significant effect on its tertiary structure and possibly modify its biological activity.
ISSN:0033-7587
1938-5404
DOI:10.2307/3577784