sup 60 Co. gamma. -rays induce predominantly C/G to G/C transversions in double-stranded M13 DNA

Upon irradiation with gamma rays of an oxygenated aqueous solution of double-stranded M13 DNA, a very specific mutation spectrum was found with respect to both the type and the positions in the DNA sequence. Of the 23 mutations, which were sequenced, 16 represent a C/G to G/C transversion. A C/G to...

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Bibliographic Details
Published in:Nucleic acids research 1988-08, Vol.16:16
Main Authors: Hoebee, B., Loman, H., Brouwer, J., van de Putte, P., Retel, J.
Format: Article
Language:English
Subjects:
560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture
AQUEOUS SOLUTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
COBALT 60
COBALT ISOTOPES
DISPERSIONS
DNA
DNA BASE TRANSITIONS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GENE MUTATIONS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
MIXTURES
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
SOLUTIONS
YEARS LIVING RADIOISOT
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Description
Summary:Upon irradiation with gamma rays of an oxygenated aqueous solution of double-stranded M13 DNA, a very specific mutation spectrum was found with respect to both the type and the positions in the DNA sequence. Of the 23 mutations, which were sequenced, 16 represent a C/G to G/C transversion. A C/G to T/A transition was found once and a G/C to T/A transversion twice. The remaining 4 mutations are frameshifts, 2 are identical and formed by the insertion of a G/C basepair; the other 2 mutations are due to a duplication of 10 basepairs situated at different positions but with a remarkable homology in base sequence. Fourteen mutations, including the 2 duplications are found in the neighborhood of a TGCT/ACGA sequence.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.16.8147