Single-sperm typing: Determination of genetic distance between the sup G. gamma. -globulin and parathyroid hormone loci by using the polymerase chain reaction and allele-specific oligomers

The frequency of recombination between the {sup G}{gamma}-globin (HBG2) and parathyroid hormone (PTH) loci on the short arm of human chromosome 11 was estimated by typing >700 single-sperm samples from two males. The sperm-typing technique employed involves the polymerase chain reaction and allel...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-12, Vol.86:23
Main Authors: Cui, Xiangfeng, Li, Honghua, Galas, D., Arnheim, N., Goradia, T.M., Lange, K., Kazazian, H.H. Jr
Format: Article
Language:English
Subjects:
550400 - Genetics
BASIC BIOLOGICAL SCIENCES
DNA HYBRIDIZATION
DNA POLYMERASES
ENZYMES
GAMETES
GENE RECOMBINATION
GENETIC MAPPING
GERM CELLS
GLOBULINS
GLOBULINS-GAMMA
HORMONES
HYBRIDIZATION
MAPPING
MATHEMATICS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
PARATHORMONE
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PROTEINS
SPERMATOZOA
STATISTICS
TRANSFERASES
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Summary:The frequency of recombination between the {sup G}{gamma}-globin (HBG2) and parathyroid hormone (PTH) loci on the short arm of human chromosome 11 was estimated by typing >700 single-sperm samples from two males. The sperm-typing technique employed involves the polymerase chain reaction and allele-specific oligonucleotide hybridization. The maximum likelihood recombination fraction estimate of 0.16 falls well within previous estimates based on family studies. With current technology and a sample size of 1000 sperm, recombination fractions down to {approx}0.009 can be estimated with statistical reliability; with a sample size of 5000 sperm, this value drops to about 0.004. Reasonable technological improvements could result in the detection of recombination frequencies
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.23.9389