Mutation Induction by Different Dose Rates of γ Rays in Radiation-Sensitive Mutants of Mouse Leukemia Cells

Induction of cell killing and mutation to 6-thioguanine resistance was examined in a radiation-sensitive mutant strain LX830 of mouse leukemia cells following γ irradiation at dose rates of 30 Gy/h (acute), 20 cGy/h (low dose rate), and 6.2 mGy/h (very low dose rate). LX830 cells were hypersensitive...

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Bibliographic Details
Published in:Radiation research 1989-11, Vol.120 (2), p.370-374
Main Authors: Furuno-Fukushi, Ikuko, Matsudaira, Hiromichi
Format: Article
Language:English
Subjects:
560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture
ANIMAL CELLS
ANIMALS
Biological and medical sciences
Biological effects of radiation
Cell culture techniques
CELL KILLING
Cell lines
Cell Survival - radiation effects
CHO cells
COBALT ISOTOPES
Cobalt Radioisotopes
Cultured cells
DISEASES
Dose-Response Relationship, Radiation
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
Flasks
Fundamental and applied biological sciences. Psychology
GAMMA RADIATION
Gamma Rays
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
IN VITRO
In Vitro Techniques
IONIZING RADIATIONS
Irradiation
ISOTOPES
LEUKEMIA
Leukemia, Experimental - genetics
Leukemia, Experimental - pathology
MAMMALS
MICE
Mutagenesis
Mutation
MUTATION FREQUENCY
MUTATIONS
NEOPLASMS
Radiation dosage
Radiation Tolerance
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
Radiotherapy
RODENTS
Short Communications
SURVIVAL TIME
Tissues, organs and organisms biophysics
TUMOR CELLS
VERTEBRATES
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Summary:Induction of cell killing and mutation to 6-thioguanine resistance was examined in a radiation-sensitive mutant strain LX830 of mouse leukemia cells following γ irradiation at dose rates of 30 Gy/h (acute), 20 cGy/h (low dose rate), and 6.2 mGy/h (very low dose rate). LX830 cells were hypersensitive to killing by acute γ rays. A slight but significant increase was observed in cell survival with decreasing dose rate down to 6.2 mGy/h, where the survival leveled off above certain total doses. The cells were also hypersensitive to mutation induction compared to the wild type. The mutation frequency increased linearly with increasing dose for all dose rates. No significant difference was observed in the frequency of induced mutations versus total dose at the three different dose rates so that the mutation frequency in LX830 cells at 6.2 mGy/h was not significantly different from that for moderate or acute irradiation.
ISSN:0033-7587
1938-5404
DOI:10.2307/3577722