Comparison of nucleoside concentrations in blood of fish with and without tumors

The objective of this study was to develop and use HPLC based analytical methodology to characterize nucleosides in blood plasma and serum from fish with and without tumors, with a goal of determining if fish blood nucleoside concentrations could similarly be used as a bioindicator of tumor developm...

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Bibliographic Details
Published in:Bulletin of environmental contamination and toxicology 1991-05, Vol.46 (5), p.713-719
Main Authors: KUEHL, D. W, EISENSCHENK, L, NAUMANN, S, JOHNSON, R. D, REGAL, R, BARNIDGE, P, MCKIM, J. JR
Format: Article
Language:English
Subjects:
560300 - Chemicals Metabolism & Toxicology
Aflatoxin B1
AFLATOXINS
Animal tumors. Experimental tumors
ANIMALS
ANTIGENS
AQUATIC ORGANISMS
Biological and medical sciences
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
CHEMICAL ANALYSIS
Experimental tumors, general aspects
Female
Fish Diseases - blood
Fish Diseases - chemically induced
FISHES
Freshwater
HAZARDOUS MATERIALS
Ictaluridae - blood
Ictalurus melas
Male
MATERIALS
Medical sciences
Neoplasms - blood
Neoplasms - veterinary
Neoplasms, Experimental - blood
Neoplasms, Experimental - chemically induced
NUCLEOSIDES
Nucleosides - blood
NUCLEOTIDES
Oncorhynchus mykiss
ORGANIC COMPOUNDS
POLLUTION
QUANTITY RATIO
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RIBOSIDES
TOXIC MATERIALS
TOXINS
TROUT
Trout - blood
Tumors
VERTEBRATES
WATER POLLUTION
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Description
Summary:The objective of this study was to develop and use HPLC based analytical methodology to characterize nucleosides in blood plasma and serum from fish with and without tumors, with a goal of determining if fish blood nucleoside concentrations could similarly be used as a bioindicator of tumor development in fish. The approach was to develop analytical methodology and quality assurance criteria for the analysis of nucleosides in fish blood, and to characterize nucleoside concentrations in blood of fish for which both healthy and tumor-bearing samples were available. Data would then be used to establish parameters with which tumor-bearing fish could be distinguished from healthy fish. Blood samples used to establish the diagnostic parameters were from control rainbow trout (Oncorhynchus mykiss) and those with tumors developed after exposure to aflatoxins. A second set of blood samples was from field collected black bullheads (Ictalurus melas).
ISSN:0007-4861
1432-0800
DOI:10.1007/BF01689957