Use of soft X-ray microscopy for analysis of early-stage biofilm formation

Soft X-ray microscopy (SXM) using synchrotron radiation is a newly-available technology for high resolution Imaging of hydrated specimens, making it potentially valuable for the study of biofilms. In this research, SXM was used to investigate bacterial spatial distribution and biofilm structure duri...

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Bibliographic Details
Published in:Water science and technology 1999, Vol.39 (7), p.269-272
Main Authors: Gilbert, E.S., Khlebnikov, A., Meyer-Ilse, W., Keasling, J.D.
Format: Article
Language:English
Subjects:
ADVANCED LIGHT SOURCE
ADVANCED LIGHT SOURCE ALS
Applied sciences
Bacteria
biofilm structure
Biofilms
Biological and medical sciences
BIOLOGICAL FOULING
Biological treatment of waters
Biotechnology
Colonization
DETECTION
Environment and pollution
ENVIRONMENTAL SCIENCES
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General purification processes
Image resolution
Image transmission
imaging
Imaging techniques
Industrial applications and implications. Economical aspects
INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
MICROSCOPY
Monolayers
Multilayers
Optics
PARTICLE ACCELERATORS
Pollution
Pseudomonas putida
Silicon nitride
SOFT X RADIATION
Soft x rays
Spatial distribution
Structure (composition)
Substrata
Synchrotron radiation
Wastewaters
Water treatment and pollution
X ray microscopes
X ray microscopy
X ray optics
X-rays
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Description
Summary:Soft X-ray microscopy (SXM) using synchrotron radiation is a newly-available technology for high resolution Imaging of hydrated specimens, making it potentially valuable for the study of biofilms. In this research, SXM was used to investigate bacterial spatial distribution and biofilm structure during the early stages of colonization of an exposed surface. Pseudomonas putida DMP-1 formed a closely-packed monolayer on a silicon nitride substratum after 24 hours of growth. The initial development of a multilayer, interlocking structure was observed. The soft X-ray images were taken with the XM-1 transmission X-ray microscope, located at the Center for X-ray Optics, Lawrence Berkeley National Laboratory. The results indicate the potential of SXM to provide new insights for the study of biofilms.
ISSN:0273-1223
1996-9732
DOI:10.1016/S0273-1223(99)00177-8