Direct fabrication of enzyme-carrying polymer nanofibers by electrospinning

Nanofibers consisting of enzyme-polymer composites have been prepared by directly electrospinning a solution of surfactant-stabilized enzyme and polymer in toluene. Additional treatment with glutaraldehyde could greatly stabilize the enzyme activity of the fibers, which could be maintained in a buff...

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Bibliographic Details
Published in:Journal of materials chemistry 2005-01, Vol.15 (31), p.3241-3245
Main Authors: HERRICKS, Thurston E, KIM, Sae-Hoon, KIM, Jungbae, DAN LI, JA HUN KWAK, GRATE, Jay W, KIM, Seong H, YOUNAN XIA
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
CHYMOTRYPSIN
Cross-disciplinary physics: materials science
rheology
Environmental Molecular Sciences Laboratory
ENZYME ACTIVITY
ENZYMES
enzymes, polymers, nanofibers, electrospinning
Exact sciences and technology
FABRICATION
LEACHING
Materials science
Physics
POLYMERS
POLYSTYRENE
SURFACTANTS
TOLUENE
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Summary:Nanofibers consisting of enzyme-polymer composites have been prepared by directly electrospinning a solution of surfactant-stabilized enzyme and polymer in toluene. Additional treatment with glutaraldehyde could greatly stabilize the enzyme activity of the fibers, which could be maintained in a buffer under shaking conditions for more than two weeks. The nanofibers also showed great improvement in the enzyme activity over bulk films as a result of increased mass-transfer for substrate molecules to and from the enzyme reactive sites. These stable and catalytically active nanofiber-based mats were highly durable and could be easily recovered from solution, making them ideal candidates for large scale applications.
ISSN:0959-9428
1364-5501
DOI:10.1039/b503660g