Characterization of Medium Conditioned by Irradiated Cells Using Proteome-Wide, High-Throughput Mass Spectrometry

Springer, D. L., Ahram, M., Adkins, J. N., Kathmann, L. E. and Miller, J. H. Characterization of Medium Conditioned by Irradiated Cells Using Proteome-Wide, High-Throughput Mass Spectrometry. Radiat. Res. 164, 651–654 (2005). Shedding, the release of cell surface proteins by regulated proteolysis, i...

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Bibliographic Details
Published in:Radiation research 2005-11, Vol.164 (5), p.651-654
Main Authors: Springer, D. L., Ahram, M., Adkins, J. N., Kathmann, L. E., Miller, J. H.
Format: Article
Language:English
Subjects:
ABUNDANCE
BASIC BIOLOGICAL SCIENCES
Breast - radiation effects
Breast - secretion
Cell membranes
Cells, Cultured
Culture Media
ENERGY TRANSFER
Environmental Molecular Sciences Laboratory
Enzymes
Epithelial Cells
Female
GROWTH FACTORS
Humans
HYPOTHESIS
Linear Energy Transfer
LYMPHOKINES
Mass Spectrometry - methods
MASS SPECTROSCOPY
Membrane proteins
Membrane Proteins - analysis
Membrane Proteins - secretion
Protein precursors
PROTEINS
PROTEOLYSIS
Proteome
Proteomics
Radiation dosage
RADIATIONS
Receptors
REGULAR ARTICLES
SENSITIVITY
Space life sciences
Tumors
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Summary:Springer, D. L., Ahram, M., Adkins, J. N., Kathmann, L. E. and Miller, J. H. Characterization of Medium Conditioned by Irradiated Cells Using Proteome-Wide, High-Throughput Mass Spectrometry. Radiat. Res. 164, 651–654 (2005). Shedding, the release of cell surface proteins by regulated proteolysis, is a general cellular response to injury and is responsible for generating numerous bioactive molecules including growth factors and cytokines. The purpose of our work is to determine whether low doses of low-linear energy transfer (LET) radiation induce shedding of bioactive molecules. Using a mass spectrometry-based global proteomics method, we tested this hypothesis by analyzing for shed proteins in medium from irradiated human mammary epithelial cells (HMEC). Several hundred proteins were identified, including transforming growth factor beta (TGFB); however, no changes in protein abundances attributable to radiation exposure, based on immunoblotting methods, were observed. These results demonstrate that our proteomic-based approach has the sensitivity to identify the kinds of proteins believed to be released after low-dose radiation exposure but that improvements in mass spectrometry-based protein quantification will be required to detect the small changes in abundance associated with this type of insult.
ISSN:0033-7587
1938-5404
DOI:10.1667/RR3457.1