Type II Cadherin Ectodomain Structures: Implications for Classical Cadherin Specificity

Type I and II classical cadherins help to determine the adhesive specificities of animal cells. Crystal-structure determination of ectodomain regions from three type II cadherins reveals adhesive dimers formed by exchange of N-terminal β strands between partner extracellular cadherin-1 (EC1) domains...

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Bibliographic Details
Published in:Cell 2006-03, Vol.124 (6), p.1255-1268
Main Authors: Patel, Saurabh D., Ciatto, Carlo, Chen, Chien Peter, Bahna, Fabiana, Rajebhosale, Manisha, Arkus, Natalie, Schieren, Ira, Jessell, Thomas M., Honig, Barry, Price, Stephen R., Shapiro, Lawrence
Format: Article
Language:English
Subjects:
ADHESIVES
Amino Acid Sequence
ANIMAL CELLS
Animals
Binding Sites
Cadherins - chemistry
Cadherins - classification
Cadherins - metabolism
Cell Line
Cells, Cultured
CHAINS
Chickens - genetics
Chickens - metabolism
CRYSTAL STRUCTURE
Crystallography, X-Ray
Dimerization
DIMERS
FUNCTIONALS
Humans
IN VITRO
IN VIVO
MATERIALS SCIENCE
Mice
Models, Molecular
national synchrotron light source
NERVE CELLS
NSLS
PARTICLE ACCELERATORS
Protein Binding
Protein Structure, Tertiary
SEGREGATION
Sequence Alignment
SPECIFICITY
Surface Properties
TRYPTOPHAN
Xenopus Proteins - genetics
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Summary:Type I and II classical cadherins help to determine the adhesive specificities of animal cells. Crystal-structure determination of ectodomain regions from three type II cadherins reveals adhesive dimers formed by exchange of N-terminal β strands between partner extracellular cadherin-1 (EC1) domains. These interfaces have two conserved tryptophan side chains that anchor each swapped strand, compared with one in type I cadherins, and include large hydrophobic regions unique to type II interfaces. The EC1 domains of type I and type II cadherins appear to encode cell adhesive specificity in vitro. Moreover, perturbation of motor neuron segregation with chimeric cadherins depends on EC1 domain identity, suggesting that this region, which includes the structurally defined adhesive interface, encodes type II cadherin functional specificity in vivo.
ISSN:0092-8674
1097-4172
DOI:10.1016/j.cell.2005.12.046