Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria

Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is ins...

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Published in:Biochemistry (Easton) 2008-12, Vol.47 (49), p.12939-12941
Main Authors: Summerfield, Tina C., Toepel, Jörg, Sherman, Louis A.
Format: Article
Language:English
Subjects:
BASIC BIOLOGICAL SCIENCES
CYANOBACTERIA
Cyanobacteria - genetics
Cyanobacteria - metabolism
Environmental Molecular Sciences Laboratory
FUNCTIONALS
Gene Expression Regulation, Bacterial
GENES
INDUCTION
Microarray Analysis
OXYGEN
Photosystem II Protein Complex - biosynthesis
Photosystem II Protein Complex - genetics
PROTEINS
RNA, Messenger - genetics
RNA, Messenger - metabolism
STRAINS
Synechocystis
Transcription, Genetic
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cited_by cdi_FETCH-LOGICAL-a475t-7b08926fe3674f49c5fe59c93d12152f16fb28a68cfa72cee52a3b35b5372a3c3
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container_end_page 12941
container_issue 49
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container_title Biochemistry (Easton)
container_volume 47
creator Summerfield, Tina C.
Toepel, Jörg
Sherman, Louis A.
description Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is inserted into functional PSII complexes. Two other cyanobacterial strains have psbA genes that were induced by low oxygen. In two of the three strains examined, psbA was part of an upregulated gene cluster including an alternative Rieske iron−sulfur protein. We conclude this cluster may represent an important adaptation to changing O2 levels that cyanobacteria experience.
doi_str_mv 10.1021/bi8018916
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects BASIC BIOLOGICAL SCIENCES
CYANOBACTERIA
Cyanobacteria - genetics
Cyanobacteria - metabolism
Environmental Molecular Sciences Laboratory
FUNCTIONALS
Gene Expression Regulation, Bacterial
GENES
INDUCTION
Microarray Analysis
OXYGEN
Photosystem II Protein Complex - biosynthesis
Photosystem II Protein Complex - genetics
PROTEINS
RNA, Messenger - genetics
RNA, Messenger - metabolism
STRAINS
Synechocystis
Transcription, Genetic
title Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria
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