A novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens

A novel fluorescence imaging technique based on deconvolution microscopy and spectral analysis is presented here as an alternative to confocal laser scanning microscopy. It allowed rapid, specific and simultaneous identification of five major opportunistic pathogens, relevant for public health, in s...

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Bibliographic Details
Published in:Journal of microbiological methods 2006-12, Vol.67 (3), p.597-602
Main Authors: Le Puil, M., Biggerstaff, J.P., Weidow, B.L., Price, J.R., Naser, S.A., White, D.C., Alberte, R.S.
Format: Article
Language:English
Subjects:
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteriological methods and techniques used in bacteriology
Bacteriology
BASIC BIOLOGICAL SCIENCES
Biological and medical sciences
Clostridium difficile - classification
Clostridium difficile - genetics
Clostridium difficile - isolation & purification
Confocal microscopy
Deconvolution
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli - isolation & purification
FISH
FLUORESCENCE
Fundamental and applied biological sciences. Psychology
INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
Listeria monocytogenes - classification
Listeria monocytogenes - genetics
Listeria monocytogenes - isolation & purification
Microbiology
MICROSCOPY
Microscopy, Fluorescence
Mycobacterium avium Complex - classification
Mycobacterium avium Complex - genetics
Mycobacterium avium Complex - isolation & purification
Mycobacterium avium subsp. paratuberculosis - classification
Mycobacterium avium subsp. paratuberculosis - genetics
Mycobacterium avium subsp. paratuberculosis - isolation & purification
PATHOGENS
QUANTITATIVE CHEMICAL ANALYSIS
RNA, Ribosomal, 16S - analysis
RNA, Ribosomal, 16S - genetics
Spectral imaging
Spectrometry, Fluorescence
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Description
Summary:A novel fluorescence imaging technique based on deconvolution microscopy and spectral analysis is presented here as an alternative to confocal laser scanning microscopy. It allowed rapid, specific and simultaneous identification of five major opportunistic pathogens, relevant for public health, in suspension and provided quantitative results.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2006.05.019