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Dual-doped thermographic phosphor particles as surrogates for GFP-labelled cells in tests of cytometric neurocatheters

We investigated the laser-induced fluorescence of particles of a compound thermographic phosphor La2O2S:Eu (1%) and Gd2O2S:Eu (1%), to see if they can serve as a surrogate for cells transfected with the green fluorescent protein, in tests of neurocatheters used for intraparenchymal cell delivery. At...

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Bibliographic Details
Published in:Journal of applied physics 2010-01, Vol.107 (6)
Main Authors: Allison, Stephen W, Gillies, George
Format: Article
Language:English
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Summary:We investigated the laser-induced fluorescence of particles of a compound thermographic phosphor La2O2S:Eu (1%) and Gd2O2S:Eu (1%), to see if they can serve as a surrogate for cells transfected with the green fluorescent protein, in tests of neurocatheters used for intraparenchymal cell delivery. At an excitation wavelength of 337 nm and a concentration of 2x106 particles ml-1, the resulting slurries produced fluorescence intensities at 625 nm that were within a factor of two of those produced by similar number densities of relevant cells, thus suggesting the utility of this approach.
ISSN:0021-8979
1089-7550
DOI:10.1063/1.3359656