Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues

Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cu...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2020-05, Vol.84 (5), p.936-942
Main Authors: Ohki, Junko, Sakashita, Akihiko, Aihara, Eitaro, Inaba, Akihiko, Uchiyama, Hironobu, Matsumoto, Masahito, Ninomiya, Yuzo, Yamane, Takumi, Oishi, Yuichi, Iwatsuki, Ken
Format: Article
Language:English
Subjects:
Animals
Basic Helix-Loop-Helix Transcription Factors - genetics
Cells, Cultured
Cholecystokinin - genetics
Cholecystokinin - metabolism
Duodenum - cytology
Enteroendocrine Cells - metabolism
Gastric Inhibitory Polypeptide - genetics
Gastric Inhibitory Polypeptide - metabolism
Ghrelin - genetics
Ghrelin - metabolism
Glucagon - genetics
Glucagon - metabolism
Glucagon-Like Peptide 1 - genetics
Glucagon-Like Peptide 1 - metabolism
Green Fluorescent Proteins - metabolism
hormone
Ileum - cytology
intestine
Jejunum - cytology
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins - genetics
Ngn3
Organoid
Organoids - cytology
RNA, Messenger - genetics
Secretin - genetics
Secretin - metabolism
Signal Transduction
Transcriptome
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Summary:Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro. Statistical analysis of genes preferentially expressed in Ngn3+ vs Ngn3- cells derived from intestinal organoids of Ngn3-EGFP mice.
ISSN:0916-8451
1347-6947
DOI:10.1080/09168451.2020.1713043