PCLN-03. ORTHOTOPIC TRANSPLANTATION OF PAEDIATRIC GLIOMA STEM CELLS IN MICE MIRRORS THE CLINICAL COURSE OF THE PATIENT

• Published in: Neuro-oncology (Charlottesville, Va.) Va.), 2018-06, Vol.20 (suppl_2), p.i155-i155
• Main Authors: Wenger, Anna, Larsson, Susanna, Dósa, Sándor, Sabel, Magnus, Kling, Teresia, Carén, Helena
• Format: Article
• Language: English
• ISSN: 1522-8517; 1523-5866
• DOI: 10.1093/neuonc/noy059.572

Abstract BACKGROUND The leading cause of cancer-related mortality among children is brain tumours and glioblastoma multiforme (GBM) has the worst prognosis. Epigenetic deregulation is believed to be a driver as these patients have few mutations compared to adults. New treatments are urgently needed but few pre-clinical in vivo models exist. One approach of generating these is to transplant tumour tissue into mice, but it yields highly variable results and requires serial passaging in mice, which is time-consuming, expensive and ethically questionable. We therefore aimed to establish a cell line-based orthotopic mouse model representative of the patient tumour. METHODS Patient-derived cancer stem cell (CSC) lines from paediatric GBM were orthotopically transplanted into immunodeficient mice. The xenograft tumours were evaluated by histology for glioma features. Genome-wide DNA methylation analysis was performed on the CSC, xenograft and patient tumours. RESULTS All CSC lines initiated tumours and the survival of the mice correlated with the survival of their respective patient. The xenograft tumours had key glioma features and were classified as GBM by histology and methylation profiling. The methylation profile of the xenograft tumour clustered together with the patient tumour (