Mass spectrometric studies of the path of carbon in photosynthesis: positional isotopic analysis of 13C-labelled C4 to C7 sugar phosphates

The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime‐ and ethoxime‐trimethylsilyl (TMS) derivatives of C4 to C7 sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled 13...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2001-05, Vol.36 (5), p.500-508
Main Authors: MacLeod, John K., Flanigan, Ian L., Williams, John F., Collins, J. Grant
Format: Article
Language:English
Subjects:
13C-labelling
alkoxime-TMS-sugars
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
GC/EIMS
isotopic analysis
Molecular biophysics
Photochemistry. Photosynthesis. Bioluminescence
Photosynthesis
Radiation-biomolecule interaction
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Summary:The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime‐ and ethoxime‐trimethylsilyl (TMS) derivatives of C4 to C7 sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled 13C analogs. In general, most but not all of the major ions in the mass spectra arise from single carbon–carbon bond cleavages of the straight‐chain derivatives. The results confirm that GC/MS of the alkoxime‐TMS derivatives is a viable method for measuring 13C incorporations at individual carbon atoms in each of the sugar phosphates during photosynthetic experiments with 13CO2. Copyright © 2001 John Wiley & Sons, Ltd.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.147