ANALYSIS OF TAXINES IN TAXUS PLANT MATERIAL AND CELL CULTURES BY HPLC PHOTODIODE ARRAY AND HPLC-ELECTROSPRAY MASS SPECTROMETRY

A semi-purified Taxus baccata needles extract was analysed by RP-HPLC. More than 18 taxines and cinnamates were detected by photodiode array detection and LC-MS, 10 of them being positively identified. Furthermore, 10-deacetyl baccatin III (paclitaxel's main precursor) and other taxanes were al...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2001-01, Vol.24 (15), p.2267-2282
Main Authors: Theodoridis, Georgios, Laskaris, Gregory, van Rozendaal, Erik L. M., Verpoorte, Robert
Format: Article
Language:English
Subjects:
Biological and medical sciences
General pharmacology
Laboratorium voor Organische chemie
Laboratory for Organic Chemistry
Medical sciences
Organic Chemistry
Organische Chemie
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
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Summary:A semi-purified Taxus baccata needles extract was analysed by RP-HPLC. More than 18 taxines and cinnamates were detected by photodiode array detection and LC-MS, 10 of them being positively identified. Furthermore, 10-deacetyl baccatin III (paclitaxel's main precursor) and other taxanes were also found in the extract. Taxines were also detected in numerous extracts of plant and cell culture extracts of various origins. The identification of the taxines in the extracts was made by correlation of retention and spectral data. LC-electrospray MS verified the identification of the known taxines in T. baccata seeds, needles, and pollen.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-100105139