Expression of cell cycle regulatory proteins in the multistep process of oesophageal carcinogenesis: stepwise over-expression of cyclin E and p53, reduction of p21WAF1/CIP1 and dysregulation of cyclin D1 and p27KIP1

• Published in: Histopathology 2001-12, Vol.39 (6), p.589-596
• Main Authors: Ohbu, M, Kobayashi, N, Okayasu, I
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; carcinogenesis; cell cycle regulatory proteins; dysplasia; Esophagus; Gastroenterology. Liver. Pancreas. Abdomen; immunohistochemistry; Medical sciences; oesophagus; Tumors
• ISSN: 0309-0167; 1365-2559
• DOI: 10.1046/j.1365-2559.2001.01279.x

Expression of cell cycle regulatory proteins in the multistep process of oesophageal carcinogenesis: stepwise over‐expression of cyclin E and p53, reduction of p21WAF1/CIP1 and dysregulation of cyclin D1 and p27KIP1 Aims: Cell cycle regulatory proteins were analysed by immunohistochemistry in order to clarify how their expression changes with the degree of atypia as oesophageal surface squamous epithelium progresses from normal mucosa, through reactive change, low‐grade dysplasia, and high‐grade dysplasia to mucosal invasive carcinoma. Methods and results: Immunostaining for cyclin D1, cyclin E, p21, p27, p53 and Ki67 proteins was performed using 22 normal mucosa, 17 reactive change, 22 low‐grade dysplasia, 15 high‐grade dysplasia and 22 mucosal invasive carcinoma specimens. Normal mucosa, low‐grade dysplasia and high‐grade dysplasia samples were taken from patients without any oesophageal invasive carcinoma by endoscopic biopsy or endoscopic mucosal resection, and reactive change and mucosal invasive carcinoma were obtained from oesophagectomy material. Stepwise over‐expression of cyclin E (P