Chemostat Approach for the Directed Evolution of Biodesulfurization Gain-of-Function Mutants

• Published in: Applied and Environmental Microbiology 2002-02, Vol.68 (2), p.691-698
• Main Authors: ARENSDORF, Joseph J, LOOMIS, A. Katrina, DIGRAZIA, Philip M, MONTICELLO, Daniel J, PIENKOS, Philip T
• Format: Article
• Language: English
• Subjects: 5-methylbenzothiophene; Bacteria; Base Sequence; Biological and medical sciences; Biotechnology; Culture Media; dszA gene; dszC gene; Evolution, Molecular; Fundamental and applied biological sciences. Psychology; Genetic technics; Genetics; Genetics and Molecular Biology; Methods. Procedures. Technologies; Microbiology; Molecular Sequence Data; Mutant screening; Mutation; octyl sulfide; Oxidoreductases - genetics; Oxidoreductases - metabolism; Oxygenases - genetics; Oxygenases - metabolism; Prokaryotes; Rhodococcus - genetics; Rhodococcus - growth & development; Rhodococcus - metabolism; Rhodococcus erythropolis; Substrate Specificity - genetics; Sulfides - chemistry; Sulfides - metabolism; Sulfur; Sulfur Compounds - metabolism; Thiophenes - chemistry; Thiophenes - metabolism
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.68.2.691-698.2002

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