UV-SPECTROPHOTOMETRIC STABILITY INDICATING METHODS FOR THE QUANTITATIVE DETERMINATION OF CIMETIDINE, FAMOTIDINE, AND RANITIDINE HYDROCHLORIDE IN THE PRESENCE OF THEIR OXIDATIVE DERIVATIVES

• Published in: Analytical letters 2002-06, Vol.35 (6), p.1055-1073
• Main Authors: Kelani, Khadiga M., Aziz, Azza M., Hegazy, Maha A., Abdel Fattah, Laila
• Format: Article
• Language: English
• Subjects: Analysis; Biological and medical sciences; Cimetidine; Famotidine; General pharmacology; Medical sciences; Pharmacology. Drug treatments; Ranitidine hydrochloride; Stability indicating method; Derivative of the ratio spectra; UV-Spectrophotometry
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1081/AL-120004555

Simple, sensitive and rapid spectrophotometric techniques have been established for the determination of cimetidine (I), famotidine (II) and ranitidine hydrochloride (III) in presence of their S-oxide derivatives in both raw materials and in pharmaceutical formulations. Hydrogen peroxide was used to enhance the formation of S-oxide compounds (oxidative derivatives). The first derivative of the ratio spectra ( 1 DD) technique has been applied for the determination of (I) and (II). While, direct zero order ( 0 D), first derivative ( 1 D) and the second derivative of the ratio spectra ( 2 DD) have been carried out for the determination of drug (III). Linear calibration curves were obtained for determination of (I) and (II) by applying 1 DD at 220 and 230 nm in the concentration range of 2-20 and 4-40 µg mL −1 with the mean percentage recoveries of 99.88 ± 0.943 (n = 10) and 99.88 ± 0.824% (n = 10) for (I) and (II), respectively. While for (III) linear calibration curves were obtained by applying 0 D, 1 D and 2 DD at 313, 334 and 255 nm, respectively. The concentration range was 2-20 µg mL −1 with the corresponding mean percentage recoveries of 100.13 ± 0.464, 100.01 ± 0.428 and 99.94 ± 0.439 for (I), (II) and (III), respectively. The results obtained by the proposed methods were statistically analyzed and compared with those obtained by the official BP methods.