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Tumor-associated Antigens as Possible Targets for Immune Therapy in Head and Neck Cancer: Comparative mRNA Expression Analysis of RAGE and GAGE Genes

Specific immune therapy targeting residual areas of cancer cells may emerge as a powerful treatment strategy for head and neck squamous cell carcinoma (HNSCC). In order to define possible targets for immune therapy, we evaluated the frequency of two groups of tumor antigens - the RAGE and GAGE famil...

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Published in:Acta oto-laryngologica 2002-01, Vol.122 (5), p.546-552
Main Authors: Götte, Karl, Usener, Dirk, Riedel, Frank, Hörmann, Karl, Schadendorf, Dirk, Eichmüller, Stefan
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cited_by cdi_FETCH-LOGICAL-c463t-fe6882f6a66cbbe9f8f9d10c80f6b9051aa65cd283092a959ab15762cc2c18a33
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container_title Acta oto-laryngologica
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creator Götte, Karl
Usener, Dirk
Riedel, Frank
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description Specific immune therapy targeting residual areas of cancer cells may emerge as a powerful treatment strategy for head and neck squamous cell carcinoma (HNSCC). In order to define possible targets for immune therapy, we evaluated the frequency of two groups of tumor antigens - the RAGE and GAGE families - by means of reverse transcriptase polymerase chain reaction using primary HNSCCs ( n =28), mucosa specimens as normal controls ( n =10) and HNSCC cell lines ( n =6). By means of specific primer selection we could differentiate between RAGE-1, -2, -3 and-4, as well as between two groups of GAGE genes (GAGE-1,2,7 vs GAGE-3,4,5,6,8). While all mucosa tissues (from smokers and non-smokers) were negative for both antigen families, 24/28 investigated tumors were positive for up to 5 tumor antigens. Among the RAGE genes, RAGE-1-positive tumors were the most abundant (8/28), followed by RAGE-2 (7/28) and RAGE-4 (6/28). Differences in the locations of HNSCCs were reflected by different RAGE family members being expressed most frequently: larynx, RAGE-1; oropharynx, RAGE-2; and hypopharynx, RAGE-4. Primers against GAGE-1,2,7 and GAGE-3,4,5,6,8 revealed 6/27 and 16/27 positive tumors, respectively. This report suggests that RAGE genes and GAGE-3,4,5,6,8 may be promising candidates for specific immune therapy in HNSCC.
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subjects Antigens
Antigens, Neoplasm
Antigens, Tumor-Associated, Carbohydrate - genetics
Antigens, Tumor-Associated, Carbohydrate - immunology
Antineoplastic agents
Associated
Biological and medical sciences
Blotting, Northern
Cancer
Carcinoma, Squamous Cell - therapy
Eye Proteins - genetics
Gage
Head
Head and Neck Neoplasms - therapy
Humans
Immune
Immunotherapy
Medical sciences
Neck
Neoplasm Proteins - genetics
Otorhinolaryngology (head neck, general aspects and miscellaneous)
Otorhinolaryngology. Stomatology
Pharmacology. Drug treatments
Rage
Receptor for Advanced Glycation End Products - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Specific
Therapy
Tumor
Tumors
title Tumor-associated Antigens as Possible Targets for Immune Therapy in Head and Neck Cancer: Comparative mRNA Expression Analysis of RAGE and GAGE Genes
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