Role of the DNA sequence downstream of the Bacillus subtilis hut promoter in regulation of the hut operon

To identify the role of the downstream region of a hut promoter in regulation of the Bacillus subtilis hut operon, three single-base substitutions (+9G to A, +14C to T, and +23T to G) were introduced into the hut operon. Analysis of expression of the hut operon containing each of these three single-...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2000-03, Vol.64 (3), p.484-491
Main Authors: Eda, S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry), Hoshino, T, Oda, M
Format: Article
Language:English
Subjects:
ADN
amino acid repression
ARN MENSAJERO
ARN MESSAGER
BACILLUS SUBTILIS
Bacillus subtilis - genetics
Bacterial Proteins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
catabolite repression
CodY
CodY protein
DNA
DNA, Bacterial
DNA-Binding Proteins
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Bacterial
Genetics
hut operon
lacZ gene
MESSENGER RNA
Microbiology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutagenesis
Mutagenesis, Site-Directed
NUCLEOTIDE SEQUENCE
Operon
Point Mutation
Promoter Regions, Genetic
Repressor Proteins - genetics
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
TRANSCRIPCION
TRANSCRIPTION
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Summary:To identify the role of the downstream region of a hut promoter in regulation of the Bacillus subtilis hut operon, three single-base substitutions (+9G to A, +14C to T, and +23T to G) were introduced into the hut operon. Analysis of expression of the hut operon containing each of these three single-base substitutions and the hut-lacZ fusions with the single-base substitutions at position +14 showed that the position at +14 and probably the position at +23 were required for amino acid repression at the hut promoter, while the position at +14 was not required for catabolite repression at the hut promoter. The position at +9 was required for a histidine-dependent increase of activity of the hut promoter. Analysis of expression of the hut-lacZ fusions and the hut operon in the codY mutant indicated that the position at +14 and probably the position at +23 were involved in CodY-mediated amino acid repression at the hut promoter and that CodY was not required for catabolite repression at t
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.64.484