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Role of the DNA sequence downstream of the Bacillus subtilis hut promoter in regulation of the hut operon
To identify the role of the downstream region of a hut promoter in regulation of the Bacillus subtilis hut operon, three single-base substitutions (+9G to A, +14C to T, and +23T to G) were introduced into the hut operon. Analysis of expression of the hut operon containing each of these three single-...
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| Published in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2000-03, Vol.64 (3), p.484-491 |
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| cites | cdi_FETCH-LOGICAL-c565t-9598863eaa39f6b56aa9a40ae01e20d9af04e2cda9df37ac4f72759f8ab50d413 |
| container_end_page | 491 |
| container_issue | 3 |
| container_start_page | 484 |
| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Eda, S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry) Hoshino, T Oda, M |
| description | To identify the role of the downstream region of a hut promoter in regulation of the Bacillus subtilis hut operon, three single-base substitutions (+9G to A, +14C to T, and +23T to G) were introduced into the hut operon. Analysis of expression of the hut operon containing each of these three single-base substitutions and the hut-lacZ fusions with the single-base substitutions at position +14 showed that the position at +14 and probably the position at +23 were required for amino acid repression at the hut promoter, while the position at +14 was not required for catabolite repression at the hut promoter. The position at +9 was required for a histidine-dependent increase of activity of the hut promoter. Analysis of expression of the hut-lacZ fusions and the hut operon in the codY mutant indicated that the position at +14 and probably the position at +23 were involved in CodY-mediated amino acid repression at the hut promoter and that CodY was not required for catabolite repression at t |
| doi_str_mv | 10.1271/bbb.64.484 |
| format | article |
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(Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry) ; Hoshino, T ; Oda, M</creator><creatorcontrib>Eda, S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry) ; Hoshino, T ; Oda, M</creatorcontrib><description>To identify the role of the downstream region of a hut promoter in regulation of the Bacillus subtilis hut operon, three single-base substitutions (+9G to A, +14C to T, and +23T to G) were introduced into the hut operon. Analysis of expression of the hut operon containing each of these three single-base substitutions and the hut-lacZ fusions with the single-base substitutions at position +14 showed that the position at +14 and probably the position at +23 were required for amino acid repression at the hut promoter, while the position at +14 was not required for catabolite repression at the hut promoter. The position at +9 was required for a histidine-dependent increase of activity of the hut promoter. Analysis of expression of the hut-lacZ fusions and the hut operon in the codY mutant indicated that the position at +14 and probably the position at +23 were involved in CodY-mediated amino acid repression at the hut promoter and that CodY was not required for catabolite repression at t</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.64.484</identifier><identifier>PMID: 10803944</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ADN ; amino acid repression ; ARN MENSAJERO ; ARN MESSAGER ; BACILLUS SUBTILIS ; Bacillus subtilis - genetics ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; catabolite repression ; CodY ; CodY protein ; DNA ; DNA, Bacterial ; DNA-Binding Proteins ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Bacterial ; Genetics ; hut operon ; lacZ gene ; MESSENGER RNA ; Microbiology ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutagenesis ; Mutagenesis, Site-Directed ; NUCLEOTIDE SEQUENCE ; Operon ; Point Mutation ; Promoter Regions, Genetic ; Repressor Proteins - genetics ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; TRANSCRIPCION ; TRANSCRIPTION</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2000-03, Vol.64 (3), p.484-491</ispartof><rights>2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2000</rights><rights>2000 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-9598863eaa39f6b56aa9a40ae01e20d9af04e2cda9df37ac4f72759f8ab50d413</citedby><cites>FETCH-LOGICAL-c565t-9598863eaa39f6b56aa9a40ae01e20d9af04e2cda9df37ac4f72759f8ab50d413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1442848$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10803944$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eda, S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry)</creatorcontrib><creatorcontrib>Hoshino, T</creatorcontrib><creatorcontrib>Oda, M</creatorcontrib><title>Role of the DNA sequence downstream of the Bacillus subtilis hut promoter in regulation of the hut operon</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>To identify the role of the downstream region of a hut promoter in regulation of the Bacillus subtilis hut operon, three single-base substitutions (+9G to A, +14C to T, and +23T to G) were introduced into the hut operon. Analysis of expression of the hut operon containing each of these three single-base substitutions and the hut-lacZ fusions with the single-base substitutions at position +14 showed that the position at +14 and probably the position at +23 were required for amino acid repression at the hut promoter, while the position at +14 was not required for catabolite repression at the hut promoter. The position at +9 was required for a histidine-dependent increase of activity of the hut promoter. Analysis of expression of the hut-lacZ fusions and the hut operon in the codY mutant indicated that the position at +14 and probably the position at +23 were involved in CodY-mediated amino acid repression at the hut promoter and that CodY was not required for catabolite repression at t</description><subject>ADN</subject><subject>amino acid repression</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>BACILLUS SUBTILIS</subject><subject>Bacillus subtilis - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>catabolite repression</subject><subject>CodY</subject><subject>CodY protein</subject><subject>DNA</subject><subject>DNA, Bacterial</subject><subject>DNA-Binding Proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetics</subject><subject>hut operon</subject><subject>lacZ gene</subject><subject>MESSENGER RNA</subject><subject>Microbiology</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Mutagenesis, Site-Directed</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Operon</subject><subject>Point Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>Repressor Proteins - genetics</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpt0M-P1CAUB3BiNO64evGuIdF4MOkI7YOW47r-zkaN0TN5pbDLhpZZaLPZ_14mnYnGyIUEPo_3-BLylLMtr1v-pu_7rYQtdHCPbHgDbSUVtPfJhikuqw4EPyGPcr5mrBwI_pCccNaxRgFsiP8Rg6XR0fnK0ndfz2i2N4udjKVDvJ3ynCyOx-u3aHwIS6Z56WcffKZXy0x3KY5xton6iSZ7uQScfZyONXsRdzbF6TF54DBk--Swn5JfH97_PP9UXXz7-Pn87KIyQoq5UkJ1nWwsYqOc7IVEVAgMLeO2ZoNCx8DWZkA1uKZFA66tW6Fch71gA_DmlLxa3y2Dla_kWY8-GxsCTjYuWfNWyIa3rMAX_8DruKSpzKY5gIJG1rIr6vWqTIo5J-v0LvkR053mTO_j1yV-LUGX-At-fnhy6Uc7_EXXvAt4eQCYDQaXcDI-_3EAdQf7pmJlfnIxjXgbUxj0jHchpmNN89_-z9Y6h1HjZSrsy_ea7Zdkpf1vyDaoEg</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Eda, S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry)</creator><creator>Hoshino, T</creator><creator>Oda, M</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope></search><sort><creationdate>20000301</creationdate><title>Role of the DNA sequence downstream of the Bacillus subtilis hut promoter in regulation of the hut operon</title><author>Eda, S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry) ; Hoshino, T ; Oda, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-9598863eaa39f6b56aa9a40ae01e20d9af04e2cda9df37ac4f72759f8ab50d413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>ADN</topic><topic>amino acid repression</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>BACILLUS SUBTILIS</topic><topic>Bacillus subtilis - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>catabolite repression</topic><topic>CodY</topic><topic>CodY protein</topic><topic>DNA</topic><topic>DNA, Bacterial</topic><topic>DNA-Binding Proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetics</topic><topic>hut operon</topic><topic>lacZ gene</topic><topic>MESSENGER RNA</topic><topic>Microbiology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Mutagenesis, Site-Directed</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Operon</topic><topic>Point Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>Repressor Proteins - genetics</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eda, S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry)</creatorcontrib><creatorcontrib>Hoshino, T</creatorcontrib><creatorcontrib>Oda, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eda, S. (Tsukuba Univ., Ibaraki (Japan). 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Analysis of expression of the hut operon containing each of these three single-base substitutions and the hut-lacZ fusions with the single-base substitutions at position +14 showed that the position at +14 and probably the position at +23 were required for amino acid repression at the hut promoter, while the position at +14 was not required for catabolite repression at the hut promoter. The position at +9 was required for a histidine-dependent increase of activity of the hut promoter. Analysis of expression of the hut-lacZ fusions and the hut operon in the codY mutant indicated that the position at +14 and probably the position at +23 were involved in CodY-mediated amino acid repression at the hut promoter and that CodY was not required for catabolite repression at t</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>10803944</pmid><doi>10.1271/bbb.64.484</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2000-03, Vol.64 (3), p.484-491 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_1442848 |
| source | EZB Free E-Journals; Oxford Journals Online |
| subjects | ADN amino acid repression ARN MENSAJERO ARN MESSAGER BACILLUS SUBTILIS Bacillus subtilis - genetics Bacterial Proteins - genetics Bacteriology Base Sequence Biological and medical sciences catabolite repression CodY CodY protein DNA DNA, Bacterial DNA-Binding Proteins Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Genetics hut operon lacZ gene MESSENGER RNA Microbiology Molecular and cellular biology Molecular genetics Molecular Sequence Data Mutagenesis Mutagenesis, Site-Directed NUCLEOTIDE SEQUENCE Operon Point Mutation Promoter Regions, Genetic Repressor Proteins - genetics SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE TRANSCRIPCION TRANSCRIPTION |
| title | Role of the DNA sequence downstream of the Bacillus subtilis hut promoter in regulation of the hut operon |
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