ESTIMATION AND ISOLATION OF AZADIRACHTIN-A FROM NEEM [AZADIRACHTA INDICA A. JUSS] SEED KERNELS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

An improved reverse phase high performance liquid chromatography (HPLC) procedure has been described to estimate azadirachtin-A content in crude methanolic extracts of neem seed kernels using valley-to-valley integration techniques. The present paper highlights a detailed study of extraction, estima...

Full description

Saved in:
Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2000-08, Vol.23 (14), p.2225-2235
Main Authors: Deota, P. T., Upadhyay, P. R., Patel, K. B., Mehta, K. J., Kamath, B. V., Mehta, M. H.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical control
Control
Fundamental and applied biological sciences. Psychology
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An improved reverse phase high performance liquid chromatography (HPLC) procedure has been described to estimate azadirachtin-A content in crude methanolic extracts of neem seed kernels using valley-to-valley integration techniques. The present paper highlights a detailed study of extraction, estimation, and purification of azadirachtin-A present in neem seed kernels. We also present, herein, our results of Soxhlet extraction of Aza-A from the defatted neem seed cake as a function of time, which is found to be more efficient than the conventional extraction procedure.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-100100484