ESTIMATION AND ISOLATION OF AZADIRACHTIN-A FROM NEEM [AZADIRACHTA INDICA A. JUSS] SEED KERNELS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

An improved reverse phase high performance liquid chromatography (HPLC) procedure has been described to estimate azadirachtin-A content in crude methanolic extracts of neem seed kernels using valley-to-valley integration techniques. The present paper highlights a detailed study of extraction, estima...

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Published in:Journal of liquid chromatography & related technologies 2000-08, Vol.23 (14), p.2225-2235
Main Authors: Deota, P. T., Upadhyay, P. R., Patel, K. B., Mehta, K. J., Kamath, B. V., Mehta, M. H.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical control
Control
Fundamental and applied biological sciences. Psychology
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
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description An improved reverse phase high performance liquid chromatography (HPLC) procedure has been described to estimate azadirachtin-A content in crude methanolic extracts of neem seed kernels using valley-to-valley integration techniques. The present paper highlights a detailed study of extraction, estimation, and purification of azadirachtin-A present in neem seed kernels. We also present, herein, our results of Soxhlet extraction of Aza-A from the defatted neem seed cake as a function of time, which is found to be more efficient than the conventional extraction procedure.
doi_str_mv 10.1081/JLC-100100484
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subjects Biological and medical sciences
Chemical control
Control
Fundamental and applied biological sciences. Psychology
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
title ESTIMATION AND ISOLATION OF AZADIRACHTIN-A FROM NEEM [AZADIRACHTA INDICA A. JUSS] SEED KERNELS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
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