Family 19 chitinase of Streptomyces griseus HUT6037 increases plant resistance to the fungal disease

Chitinase C (ChiC) is the first bacterial family 19 chitinase discovered in Streptomyces griseus HUT6037. In vitro, ChiC clearly inhibited hyphal extension of Trichoderma reesei but a rice family 19 chitinase did not. In order to investigate the effects of ChiC as an increaser of plant resistance to...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-04, Vol.67 (4), p.847-855
Main Authors: Itoh, Y. (Niigata Univ. (Japan)), Takahashi, K, Takizawa, H, Nikaidou, N, Tanaka, H, Nishihashi, H, Watanabe, T, Nishizawa, Y
Format: Article
Language:English
Subjects:
antifungal activity
Antifungal Agents - pharmacology
ANTIFUNGAL PROPERTIES
Biological and medical sciences
CHITINASE
Chitinases - genetics
Chitinases - pharmacology
DISEASE RESISTANCE
family 19 chitinase
Fundamental and applied biological sciences. Psychology
FUNGAL DISEASES
Genetic Vectors
GROWTH INHIBITORS
Immunity - genetics
Magnaporthe
MAGNAPORTHE GRISEA
MYCELIUM
Mycoses - prevention & control
Oryza
ORYZA SATIVA
Plant Diseases - microbiology
Plant Proteins
Plants, Genetically Modified - microbiology
STREPTOMYCES GRISEUS
Streptomyces griseus - enzymology
TRANSGENIC PLANTS
transgenic rice
Trichoderma - drug effects
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Summary:Chitinase C (ChiC) is the first bacterial family 19 chitinase discovered in Streptomyces griseus HUT6037. In vitro, ChiC clearly inhibited hyphal extension of Trichoderma reesei but a rice family 19 chitinase did not. In order to investigate the effects of ChiC as an increaser of plant resistance to fungal diseases, the chiC gene was introduced into rice plants under the control of the increased CaMV 35S promoter and a signal sequence from the rice chitinase gene. Transgenic plants were morphologically normal. Resistance to leaf blast disease caused by Magnaporthe grisea was evaluated in R 1 and R 2 generations using a spray method. Ninety percent of transgenic rice plants expressing ChiC had higher resistance than non-transgenic plants. Disease resistance of sibling plants within the same line was correlated with the ChiC expression levels. ChiC produced in rice plants accumulated intercellularly and had the hydrolyzing activity against glycol chitin.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.67.847