Effect of parental genotypes and colchicine treatment on the androgenic response of wheat F1 hybrids

• Published in: Plant breeding 2003-08, Vol.122 (4), p.314-317
• Main Authors: Zamani, I, Gouli-Vavdinoudi, E, Kovacs, G, Xynias, I, Roupakias, D, Barnabas, B
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; androgenesis; anther culture; Biological and medical sciences; colchicine; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; genotype; Haploidy, in vitro culture applications, somatic hybrids; hybrid parental genotype; hybridization; in vitro regeneration; plant breeding; Plant breeding: fundamental aspects and methodology; Triticum aestivum; varieties; wheat
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1046/j.1439-0523.2003.00866.x

The effect of the parental genotypes and colchicine treatment on the androgenic response of wheat (Triticum aestivum L.) F1 hybrids was studied. For this, anthers from three F1 hybrids and their parents were cultured on W14 initiation medium and W14 supplemented with 0.03% colchicine. The number of responding anthers, microspore‐derived structures/100 anthers, green plants/embryos cultured, green plants/100 anthers and albino plants/100 anthers were recorded. It was observed that embryo formation and plant regeneration ability were genetically controlled and genotype dependent. In both treatments the variety Kavkaz had a significantly higher percentage of responding anthers, microspore‐derived structures and green plants/100 anthers than the other genotypes. On the other hand, the variety Myconos also demonstrated high microspore‐derived structure production and green plant regeneration when treated with colchicine. The good response observed in these two varieties indicates the importance of colchicine treatment only for certain genotypes. Green plant production capacity of the hybrids was intermediate to that of the parental varieties. As one parent with a high or even an intermediate response to anther culture could lead to the production of sufficient (for breeding purposes) green plants from the F1 hybrids, it was concluded that screening the inbred lines for the response to anther culture with and without colchicine treatment could contribute to utilization of breeding material with a low response to anther culture via the proper hybrid combinations.