Kinetic studies of glutathione S-transferase-catalysed processes through on-line liquid chromatography-electrospray mass spectrometry

Mass spectrometry is a sensitive and accurate technique for the direct monitoring of enzyme-catalysed reactions that does not require a chromophore or radiolabelling and thus provides a reliable alternative to the existing analytical approaches. In this study, the on-line technique high-pressure liq...

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Bibliographic Details
Published in:Philosophical magazine (2003. Print) 2004-05, Vol.84 (13-16), p.1373-1382
Main Authors: Rossi, B., Mancini, I., Guella, G., Viliani, G.
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Exact sciences and technology
Mass spectrometry (including SIMS, multiphoton ionization and resonance ionization mass spectrometry)
Spectrometric and optical methods
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Summary:Mass spectrometry is a sensitive and accurate technique for the direct monitoring of enzyme-catalysed reactions that does not require a chromophore or radiolabelling and thus provides a reliable alternative to the existing analytical approaches. In this study, the on-line technique high-pressure liquid chromatography (LC)-electrospray ionization (ESI) has been applied to the glutathione S-transferase-catalysed dehalogenation of haloaromatic compounds with the tripeptide glutathione, a process playing a central role in the catabolism of aromatic substrates in all living organisms. Attention has been focused on the relative leaving group mobility order of the halogen groups in benzene and napthalene systems.
ISSN:1478-6435
1478-6443
DOI:10.1080/14786430310001644116