cDNA cloning characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca

A secreted luciferase from the marine ostracod, Vargula hilgendorfii, is a useful tool for gene expression assays in living mammalian cells. We have cloned the cDNA of a new secreted luciferase from the ostracod Cypridina noctiluca, which inhabits the coast of Japan. C. noctiluca luciferase consists...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-03, Vol.68 (3), p.565-570
Main Authors: Nakajima, Y. (National Inst. of Advanced Industrial Science and Technology Kansai, Ikeda, Osaka (Japan)), Kobayashi, K, Yamagishi, K, Enomoto, T, Ohmiya, Y
Format: Article
Language:English
Subjects:
Amino Acid Sequence
AMINO ACID SEQUENCES
Animals
Biological and medical sciences
bioluminescence
CELL CULTURE
Cell Culture Techniques
Cloning, Molecular
Crustacea - enzymology
Crustacea - genetics
ENZYME ACTIVITY
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Gene Library
GENES
luciferase
Luciferases - genetics
Luciferases - metabolism
LUMINESCENCE
Marine
MOLECULAR CLONING
Molecular Sequence Data
ostracod
OSTRACODA
OXIDOREDUCTASES
reporter gene
SECRETION
Sequence Alignment
Transfection
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Summary:A secreted luciferase from the marine ostracod, Vargula hilgendorfii, is a useful tool for gene expression assays in living mammalian cells. We have cloned the cDNA of a new secreted luciferase from the ostracod Cypridina noctiluca, which inhabits the coast of Japan. C. noctiluca luciferase consists of 553 amino acid residues with a molecular mass of 61,415 Da, as deduced from the nucleotide sequence. The homologies of nucleotide and amino acid sequences with V. hilgendorfii luciferase are 79.2% and 83.1%, respectively. C. noctiluca luciferase can expressed in and secreted from cultured mammalian cells. The characteristic properties of expressed C. noctiluca luciferase are similar to those of V. hilgendorfii luciferase. However, the activity of C. noctiluca luciferase in culture medium is much higher than that of V. hilgendorfii luciferase, suggesting that C. noctiluca luciferase is a highly potent reporter enzyme for real-time and continuous monitoring of gene expression in living cells.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.68.565