LC Determination of the Enantiomeric Purity of L-Arginine Using a Teicoplanin Chiral Stationary Phase

A direct enantioselective high-performance liquid chromatography (HPLC) method was employed successfully for determination of the enantiomeric purity of L-arginine. The elaborated method used teicoplanin macrocyclic antibiotic chiral stationary phase (CSP), known as Chirobiotic T, with a reversed-ph...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2004-01, Vol.27 (11), p.1681-1693
Main Authors: Aboul-Enein, Hassan Y., Hefnawy, Mohamed M., Hoenen, Hubert
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chiral HPLC
Chirobiotic T
Chromatographic methods and physical methods associated with chromatography
Enantiomeric purity
Exact sciences and technology
L-Arginine
Other chromatographic methods
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Summary:A direct enantioselective high-performance liquid chromatography (HPLC) method was employed successfully for determination of the enantiomeric purity of L-arginine. The elaborated method used teicoplanin macrocyclic antibiotic chiral stationary phase (CSP), known as Chirobiotic T, with a reversed-phase mobile phase consisting of methanol:50 mM sodium dihydrogen phosphate buffer, pH 4.6 (2:8, v/v), at a flow rate of 1.0 mL/min and UV detection at 214 nm. Linearity, precision, accuracy, and the quantitation limit were determined. The method proved to be capable of determining 0.0025% (w/w) of D-arginine (the enantiomeric impurity) contrary to the pharmacopoeial limit measurement, in which only amounts of D-arginine higher than 1.5% (w/w) caused the measurement to fail.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-120037364