Expression of the pMGA genes of Mycoplasma gallisepticum is controlled by variation in the GAA trinucleotide repeat lengths within the 5' noncoding regions

We analyzed the segment of DNA which contains the expressed pMGA gene from one strain of Mycoplasma gallisepticum in normal (strain S6) cells and in cells in which pMGA1.1 gene expression had ceased as a consequence of in vitro culture in the presence of pMGA1.1-specific antibodies. Sequence analysi...

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Bibliographic Details
Published in:Infection and Immunity 1998-12, Vol.66 (12), p.5833-5841
Main Authors: Glew, M.D, Baseggio, N, Markham, P.F, Browning, G.F, Walker, I.D
Format: Article
Language:English
Subjects:
Bacterial Proteins - biosynthesis
Bacterial Proteins - genetics
Bacteriology
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gene Dosage
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genetics
Lipoproteins - biosynthesis
Lipoproteins - genetics
messenger RNA
Microbiology
Molecular and Cellular Pathogenesis
Molecular Sequence Data
Mycoplasma - genetics
Mycoplasma gallisepticum
nucleotide sequences
Promoter Regions, Genetic
Trinucleotide Repeat Expansion
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Summary:We analyzed the segment of DNA which contains the expressed pMGA gene from one strain of Mycoplasma gallisepticum in normal (strain S6) cells and in cells in which pMGA1.1 gene expression had ceased as a consequence of in vitro culture in the presence of pMGA1.1-specific antibodies. Sequence analysis of isolates lacking pMGA1.1 expression revealed that this gene, which is typically expressed, exhibited sequence changes within a region 5' to its promoter. Specifically, pMGA1.1+ cells contained a (GAA)12 motif upstream of the promoter, whereas in pMGA1.1- cells the corresponding region contained a (GAA)10 motif, when such cells were grown in medium no longer containing pMGA-specific antibodies, pMGA1.1 was reexpressed and the 5' (GAA)12 motif was restored. Two other genes, pMGA1.9 and pMGA1.2, were also shown to acquire a (GAA)12 motif in clones which expressed these genes. The results imply the evolution by the pMGA genes of M. gallisepticum of a novel transcriptional requirement which facilitates rapid and reversible switches in the pMGA expression pattern.
ISSN:0019-9567
1098-5522
DOI:10.1128/iai.66.12.5833-5841.1998