Seroepidemiology of Human Metapneumovirus (hMPV) on the Basis of a Novel Enzyme-Linked Immunosorbent Assay Utilizing hMPV Fusion Protein Expressed in Recombinant Vesicular Stomatitis Virus

Human metapneumovirus (hMPV) is a newly identified human respiratory virus now recognized as a major respiratory pathogen of infants and children. To define the seroepidemiology of hMPV, we developed a novel enzyme-linked immunosorbent assay (ELISA) based on expression of the fusion protein of hMPV...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-03, Vol.43 (3), p.1213-1219
Main Authors: Leung, Jessica, Esper, Frank, Weibel, Carla, Kahn, Jeffrey S
Format: Article
Language:English
Subjects:
Adolescent
Antibodies, Viral - blood
Biological and medical sciences
Child
Child, Preschool
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Human metapneumovirus
Humans
Infant
Infant, Newborn
Infectious diseases
Medical sciences
Metapneumovirus - immunology
Microbiology
Miscellaneous
Paramyxoviridae Infections - epidemiology
Paramyxovirus
Recombinant Proteins - immunology
Respiratory syncytial virus
Seroepidemiologic Studies
Vesicular stomatitis Indiana virus - genetics
Vesicular stomatitis virus
Viral Fusion Proteins - genetics
Viral Fusion Proteins - immunology
Virology
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Summary:Human metapneumovirus (hMPV) is a newly identified human respiratory virus now recognized as a major respiratory pathogen of infants and children. To define the seroepidemiology of hMPV, we developed a novel enzyme-linked immunosorbent assay (ELISA) based on expression of the fusion protein of hMPV (hMPV F) in recombinant vesicular stomatitis virus (VSV). Western blot analysis using an hMPV F-specific antiserum confirmed the expression of hMPV in recombinant VSV. The ELISA is specific for hMPV F; antibody specific for the most closely related human paramyxovirus, respiratory syncytial virus, does not bind to hMPV F. Overall, 216 serum specimens were tested. The percentages of seropositive individuals were 89.1% in children
ISSN:0095-1137
1098-660X
DOI:10.1128/jcm.43.3.1213-1219.2005