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Seroepidemiology of Human Metapneumovirus (hMPV) on the Basis of a Novel Enzyme-Linked Immunosorbent Assay Utilizing hMPV Fusion Protein Expressed in Recombinant Vesicular Stomatitis Virus
Human metapneumovirus (hMPV) is a newly identified human respiratory virus now recognized as a major respiratory pathogen of infants and children. To define the seroepidemiology of hMPV, we developed a novel enzyme-linked immunosorbent assay (ELISA) based on expression of the fusion protein of hMPV...
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| Published in: | Journal of Clinical Microbiology 2005-03, Vol.43 (3), p.1213-1219 |
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| container_issue | 3 |
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| container_title | Journal of Clinical Microbiology |
| container_volume | 43 |
| creator | Leung, Jessica Esper, Frank Weibel, Carla Kahn, Jeffrey S |
| description | Human metapneumovirus (hMPV) is a newly identified human respiratory virus now recognized as a major respiratory pathogen of infants and children. To define the seroepidemiology of hMPV, we developed a novel enzyme-linked immunosorbent assay (ELISA) based on expression of the fusion protein of hMPV (hMPV F) in recombinant vesicular stomatitis virus (VSV). Western blot analysis using an hMPV F-specific antiserum confirmed the expression of hMPV in recombinant VSV. The ELISA is specific for hMPV F; antibody specific for the most closely related human paramyxovirus, respiratory syncytial virus, does not bind to hMPV F. Overall, 216 serum specimens were tested. The percentages of seropositive individuals were 89.1% in children |
| doi_str_mv | 10.1128/jcm.43.3.1213-1219.2005 |
| format | article |
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To define the seroepidemiology of hMPV, we developed a novel enzyme-linked immunosorbent assay (ELISA) based on expression of the fusion protein of hMPV (hMPV F) in recombinant vesicular stomatitis virus (VSV). Western blot analysis using an hMPV F-specific antiserum confirmed the expression of hMPV in recombinant VSV. The ELISA is specific for hMPV F; antibody specific for the most closely related human paramyxovirus, respiratory syncytial virus, does not bind to hMPV F. Overall, 216 serum specimens were tested. The percentages of seropositive individuals were 89.1% in children </=5 months old, 55.0% in children 6 to 11 months old, 36.0% in children 12 to 23 months old, 45.0% in children 24 to 47 months old, 77.3% in children 48 to 59 months old, 91.3% in children 5 to 10 years old, and 95.5% for individuals 11 to 20 years old. This is the first seroepidemiological survey of hMPV in the United States and the first analysis to determine the prevalence of antibody to a specific hMPV protein. The data suggest that exposure to hMPV is common in childhood and that hMPV F is an antigenic determinant of hMPV.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.43.3.1213-1219.2005</identifier><identifier>PMID: 15750086</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Adolescent ; Antibodies, Viral - blood ; Biological and medical sciences ; Child ; Child, Preschool ; Cloning, Molecular ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; Human metapneumovirus ; Humans ; Infant ; Infant, Newborn ; Infectious diseases ; Medical sciences ; Metapneumovirus - immunology ; Microbiology ; Miscellaneous ; Paramyxoviridae Infections - epidemiology ; Paramyxovirus ; Recombinant Proteins - immunology ; Respiratory syncytial virus ; Seroepidemiologic Studies ; Vesicular stomatitis Indiana virus - genetics ; Vesicular stomatitis virus ; Viral Fusion Proteins - genetics ; Viral Fusion Proteins - immunology ; Virology</subject><ispartof>Journal of Clinical Microbiology, 2005-03, Vol.43 (3), p.1213-1219</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005, American Society for Microbiology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-d0c5b12963c510b1892d9db7c6f190a43c542f26fd676bd0198b544caaef51a93</citedby><cites>FETCH-LOGICAL-c592t-d0c5b12963c510b1892d9db7c6f190a43c542f26fd676bd0198b544caaef51a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1081231/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1081231/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,3175,3176,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16612078$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15750086$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leung, Jessica</creatorcontrib><creatorcontrib>Esper, Frank</creatorcontrib><creatorcontrib>Weibel, Carla</creatorcontrib><creatorcontrib>Kahn, Jeffrey S</creatorcontrib><title>Seroepidemiology of Human Metapneumovirus (hMPV) on the Basis of a Novel Enzyme-Linked Immunosorbent Assay Utilizing hMPV Fusion Protein Expressed in Recombinant Vesicular Stomatitis Virus</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>Human metapneumovirus (hMPV) is a newly identified human respiratory virus now recognized as a major respiratory pathogen of infants and children. To define the seroepidemiology of hMPV, we developed a novel enzyme-linked immunosorbent assay (ELISA) based on expression of the fusion protein of hMPV (hMPV F) in recombinant vesicular stomatitis virus (VSV). Western blot analysis using an hMPV F-specific antiserum confirmed the expression of hMPV in recombinant VSV. The ELISA is specific for hMPV F; antibody specific for the most closely related human paramyxovirus, respiratory syncytial virus, does not bind to hMPV F. Overall, 216 serum specimens were tested. The percentages of seropositive individuals were 89.1% in children </=5 months old, 55.0% in children 6 to 11 months old, 36.0% in children 12 to 23 months old, 45.0% in children 24 to 47 months old, 77.3% in children 48 to 59 months old, 91.3% in children 5 to 10 years old, and 95.5% for individuals 11 to 20 years old. This is the first seroepidemiological survey of hMPV in the United States and the first analysis to determine the prevalence of antibody to a specific hMPV protein. The data suggest that exposure to hMPV is common in childhood and that hMPV F is an antigenic determinant of hMPV.</description><subject>Adolescent</subject><subject>Antibodies, Viral - blood</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cloning, Molecular</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Human metapneumovirus</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Metapneumovirus - immunology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Paramyxoviridae Infections - epidemiology</subject><subject>Paramyxovirus</subject><subject>Recombinant Proteins - immunology</subject><subject>Respiratory syncytial virus</subject><subject>Seroepidemiologic Studies</subject><subject>Vesicular stomatitis Indiana virus - genetics</subject><subject>Vesicular stomatitis virus</subject><subject>Viral Fusion Proteins - genetics</subject><subject>Viral Fusion Proteins - immunology</subject><subject>Virology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFks1uEzEUhUcIREvhFahZgGAxwdfz6w1SiVJalEBFSMTO8ng8iduxHeyZQPpsPBweJSKwYmPL9nfOvVc-UXQOeARAyre3Qo_SZJSMgEASh4WOCMbZg-gUMC3jPMffHkanGNMsBkiKk-iJ97cYQ5pm2ePoBLIiw7jMT6Nfc-ms3KhaamVbu9oh26CrXnODZrLjGyN7bbfK9R69Xs9ulm-QNahbS_See-UHmKNPditbNDH3Oy3jqTJ3skbXWvfGeusqaTp04T3foUWnWnWvzAoNTuiy9yqY3TjbSWXQ5OfGSe-DNhy-SGF1pQwP4qX0SvQtd2jeWc071YXCy6Glp9GjhrdePjvsZ9HicvJ1fBVPP3-4Hl9MY5FR0sU1FlkFhOaJyABXUFJS07oqRN4AxTwN1ylpSN7UeZFXNQZaVlmaCs5lkwGnyVn0bu-76SstaxFGcrxlG6c0dztmuWL_vhi1Ziu7ZYBLIAkEg1cHA2e_99J3TCsvZNtyI23vWV6kZRk-8b8gFCXkJSYBLPagcNZ7J5s_3QBmQ0TYx_GMpQlL2BCRYaFsiEhQPv97mKPukIkAvDwA3AveNo4bofyRy3MguCgD92LPrdVq_UM5ybjXLCTzWDYw53um4ZbxlQs-iznBkIRo0gJTSH4D5P3eMg</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>Leung, Jessica</creator><creator>Esper, Frank</creator><creator>Weibel, Carla</creator><creator>Kahn, Jeffrey S</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050301</creationdate><title>Seroepidemiology of Human Metapneumovirus (hMPV) on the Basis of a Novel Enzyme-Linked Immunosorbent Assay Utilizing hMPV Fusion Protein Expressed in Recombinant Vesicular Stomatitis Virus</title><author>Leung, Jessica ; Esper, Frank ; Weibel, Carla ; Kahn, Jeffrey S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-d0c5b12963c510b1892d9db7c6f190a43c542f26fd676bd0198b544caaef51a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adolescent</topic><topic>Antibodies, Viral - blood</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cloning, Molecular</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Human metapneumovirus</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Metapneumovirus - immunology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Paramyxoviridae Infections - epidemiology</topic><topic>Paramyxovirus</topic><topic>Recombinant Proteins - immunology</topic><topic>Respiratory syncytial virus</topic><topic>Seroepidemiologic Studies</topic><topic>Vesicular stomatitis Indiana virus - genetics</topic><topic>Vesicular stomatitis virus</topic><topic>Viral Fusion Proteins - genetics</topic><topic>Viral Fusion Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leung, Jessica</creatorcontrib><creatorcontrib>Esper, Frank</creatorcontrib><creatorcontrib>Weibel, Carla</creatorcontrib><creatorcontrib>Kahn, Jeffrey S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leung, Jessica</au><au>Esper, Frank</au><au>Weibel, Carla</au><au>Kahn, Jeffrey S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seroepidemiology of Human Metapneumovirus (hMPV) on the Basis of a Novel Enzyme-Linked Immunosorbent Assay Utilizing hMPV Fusion Protein Expressed in Recombinant Vesicular Stomatitis Virus</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>43</volume><issue>3</issue><spage>1213</spage><epage>1219</epage><pages>1213-1219</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>Human metapneumovirus (hMPV) is a newly identified human respiratory virus now recognized as a major respiratory pathogen of infants and children. To define the seroepidemiology of hMPV, we developed a novel enzyme-linked immunosorbent assay (ELISA) based on expression of the fusion protein of hMPV (hMPV F) in recombinant vesicular stomatitis virus (VSV). Western blot analysis using an hMPV F-specific antiserum confirmed the expression of hMPV in recombinant VSV. The ELISA is specific for hMPV F; antibody specific for the most closely related human paramyxovirus, respiratory syncytial virus, does not bind to hMPV F. Overall, 216 serum specimens were tested. The percentages of seropositive individuals were 89.1% in children </=5 months old, 55.0% in children 6 to 11 months old, 36.0% in children 12 to 23 months old, 45.0% in children 24 to 47 months old, 77.3% in children 48 to 59 months old, 91.3% in children 5 to 10 years old, and 95.5% for individuals 11 to 20 years old. This is the first seroepidemiological survey of hMPV in the United States and the first analysis to determine the prevalence of antibody to a specific hMPV protein. The data suggest that exposure to hMPV is common in childhood and that hMPV F is an antigenic determinant of hMPV.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>15750086</pmid><doi>10.1128/jcm.43.3.1213-1219.2005</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of Clinical Microbiology, 2005-03, Vol.43 (3), p.1213-1219 |
| issn | 0095-1137 1098-660X |
| language | eng |
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| source | American Society for Microbiology Journals; PubMed Central |
| subjects | Adolescent Antibodies, Viral - blood Biological and medical sciences Child Child, Preschool Cloning, Molecular Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Human metapneumovirus Humans Infant Infant, Newborn Infectious diseases Medical sciences Metapneumovirus - immunology Microbiology Miscellaneous Paramyxoviridae Infections - epidemiology Paramyxovirus Recombinant Proteins - immunology Respiratory syncytial virus Seroepidemiologic Studies Vesicular stomatitis Indiana virus - genetics Vesicular stomatitis virus Viral Fusion Proteins - genetics Viral Fusion Proteins - immunology Virology |
| title | Seroepidemiology of Human Metapneumovirus (hMPV) on the Basis of a Novel Enzyme-Linked Immunosorbent Assay Utilizing hMPV Fusion Protein Expressed in Recombinant Vesicular Stomatitis Virus |
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