IrgA Homologue Adhesin Iha Is an Escherichia coli Virulence Factor in Murine Urinary Tract Infection

The role of the Escherichia coli iron-regulated gene homologue adhesin (Iha) in the pathogenesis of urinary tract infections (UTIs) is unknown. We performed a series of complementary analyses to confirm or refute the hypothesis that Iha is a virulence factor in uropathogenic E. coli. Fecal E. coli i...

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Bibliographic Details
Published in:Infection and Immunity 2005-02, Vol.73 (2), p.965-971
Main Authors: Johnson, James R, Jelacic, Srdjan, Schoening, Laura M, Clabots, Connie, Shaikh, Nurmohammad, Mobley, Harry L. T, Tarr, Phillip I
Format: Article
Language:English
Subjects:
Adhesins, Escherichia coli - genetics
Adhesins, Escherichia coli - immunology
Adhesins, Escherichia coli - metabolism
Animals
Bacterial Infections
Bacteriology
Biological and medical sciences
Epithelial Cells - immunology
Epithelial Cells - metabolism
Epithelial Cells - microbiology
Escherichia coli
Escherichia coli - immunology
Escherichia coli - metabolism
Escherichia coli - pathogenicity
Female
Fundamental and applied biological sciences. Psychology
Humans
Mice
Microbiology
Miscellaneous
Molecular Sequence Data
Urinary Tract Infections - immunology
Urinary Tract Infections - metabolism
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Summary:The role of the Escherichia coli iron-regulated gene homologue adhesin (Iha) in the pathogenesis of urinary tract infections (UTIs) is unknown. We performed a series of complementary analyses to confirm or refute the hypothesis that Iha is a virulence factor in uropathogenic E. coli. Fecal E. coli isolates exhibited significantly lower prevalences of iha (range, 14 to 22%) than did clinical isolates from cases of pediatric cystitis or pyelonephritis, adult pyelonephritis or urosepsis, or bacteremia (range, 38 to 74%). Recombinant Iha from E. coli pyelonephritis isolate CFT073 conferred upon nonadherent E. coli ORN172 the ability to adhere to cultured T-24 human uroepithelial cells. In a well-established mouse model of ascending UTI, CFT073 and its derivative UPEC76 (a pap [P fimbriae] mutant version of strain CFT073) each significantly outcompeted their respective iha deletion mutants in CBA/J mice 48 h after bladder challenge (P < 0.03 for urine, both kidneys, and bladders of both constructs, except for bladders of mice challenged with UPEC76 and its deletion mutant, where P = 0.11). These data suggest that Iha[subscript CFT073] is a virulence factor and might be a target for anti-UTI interventions.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.73.2.965-971.2005