Lactococcus lactis SpOx Spontaneous Mutants: a Family of Oxidative-Stress-Resistant Dairy Strains

Numerous industrial bacteria generate hydrogen peroxide (H₂O₂), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medi...

Full description

Saved in:
Bibliographic Details
Published in:Applied and Environmental Microbiology 2005-05, Vol.71 (5), p.2782-2788
Main Authors: Rochat, Tatiana, Gratadoux, Jean-Jacques, Corthier, Gérard, Coqueran, Bérard, Nader-Macias, Maria-Elena, Gruss, Alexandra, Langella, Philippe
Format: Article
Language:English
Subjects:
animal models
Animals
Bacteria
Biological and medical sciences
biological resistance
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
cell culture
cultured milk starters
Ecology, environment
Food science
Fundamental and applied biological sciences. Psychology
Humans
hydrogen peroxide
Hydrogen Peroxide - pharmacology
intestinal microorganisms
intestines
Isolation and description
Lactobacillus delbrueckii
Lactobacillus delbrueckii subsp. delbrueckii
Lactococcus lactis
Lactococcus lactis - drug effects
Lactococcus lactis - genetics
Lactococcus lactis - growth & development
Lactococcus lactis - metabolism
Life Sciences
Mice
microbial growth
Microbiology
Milk
Milk - microbiology
Mission oriented research
mutants
Mutation
natural selection
Oxidative Stress
Physiology and Biotechnology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Numerous industrial bacteria generate hydrogen peroxide (H₂O₂), which may inhibit the growth of other bacteria in mixed ecosystems. We isolated spontaneous oxidative-stress-resistant (SpOx) Lactococcus lactis mutants by using a natural selection method with milk-adapted strains on dairy culture medium containing H₂O₂. Three SpOx mutants displayed greater H₂O₂ resistance. One of them, SpOx3, demonstrated better behavior in different oxidative-stress situations: (i) higher long-term survival upon aeration in LM17 and milk and (ii) the ability to grow with H₂O₂-producing Lactobacillus delbrueckii subsp. delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.71.5.2782-2788.2005