Determination of Hydrogen Peroxide, Glucose and Hypoxanthine using (Bio)Sensors Based on Ruthenium Dioxide-Modified Screen-Printed Electrodes

Screen-printed electrodes modified with ruthenium dioxide were investigated as amperometric sensors for the determination of hydrogen peroxide using flow injection analysis (FIA). With additions of enzymes (glucose oxidase or xanthine oxidase) immobilized onto the electrode surface via Nafion films,...

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Bibliographic Details
Published in:Analytical letters 2005-05, Vol.38 (7), p.1099-1113
Main Authors: Kotzian, Petr, Brázdilová, Petra, Kalcher, Kurt, Vytřas, Karel
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
biosensor
Biosensors
Biotechnology
Chemistry
Electrochemical methods
Exact sciences and technology
flow injection analysis
Fundamental and applied biological sciences. Psychology
General, instrumentation
glucose
hydrogen peroxide
hypoxanthine
Methods. Procedures. Technologies
modification
ruthenium dioxide
Screen-printed electrodes
Various methods and equipments
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Summary:Screen-printed electrodes modified with ruthenium dioxide were investigated as amperometric sensors for the determination of hydrogen peroxide using flow injection analysis (FIA). With additions of enzymes (glucose oxidase or xanthine oxidase) immobilized onto the electrode surface via Nafion films, corresponding biosensors for the quantification of glucose or hypoxanthine were developed. Factors influencing their amperometric responses (operational potential, flow rate, pH of the phosphate buffer carrier) were studied in detail. Applications in food analysis were also examined. Supported by the Ministry of Education, Youth, and Sport of the Czech Republic (project MSM0021627502) and from the Grant Agency of the Czech Republic (No. 203/05/2106). Partial support of the CEEPUS program (project PL-110) is also gratefully acknowledged.
ISSN:0003-2719
1532-236X
DOI:10.1081/AL-200057205