Immunohistological Characterization of Macrophage Migration Inhibitory Factor Expression in Plasmodium falciparum-Infected Placentas

Previously, we have shown that macrophage migration inhibitory factor (MIF) was highly elevated in the placental intervillous blood (IVB) of Plasmodium falciparum-infected women. Here, we compared the expression of MIF in placental tissues obtained from P. falciparum-infected and -uninfected women....

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Published in:Infection and Immunity 2005-06, Vol.73 (6), p.3287-3293
Main Authors: Chaisavaneeyakorn, Sujittra, Lucchi, Naomi, Abramowsky, Carlos, Othoro, Caroline, Chaiyaroj, Sansanee C, Shi, Ya Ping, Nahlen, Bernard L, Peterson, David S, Moore, Julie M, Udhayakumar, Venkatachalam
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
Choriocarcinoma - metabolism
Female
Fundamental and applied biological sciences. Psychology
Fungal and Parasitic Infections
Humans
Immunohistochemistry
Leukocytes, Mononuclear - chemistry
Macrophage Migration-Inhibitory Factors - analysis
Macrophage Migration-Inhibitory Factors - biosynthesis
Microbiology
Placenta - chemistry
Placenta - parasitology
Plasmodium falciparum
Plasmodium falciparum - pathogenicity
Pregnancy
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Summary:Previously, we have shown that macrophage migration inhibitory factor (MIF) was highly elevated in the placental intervillous blood (IVB) of Plasmodium falciparum-infected women. Here, we compared the expression of MIF in placental tissues obtained from P. falciparum-infected and -uninfected women. Immunoperoxidase staining showed a consistent pattern of MIF expression in syncytiotrophoblasts, extravillous trophoblasts, IVB mononuclear cells, and amniotic epithelial cells, irrespective of their malaria infection status. Cytotrophoblast, villous stroma, and Hofbauer cells showed focal staining. Only amniotic epithelial and IVB mononuclear cells from P. falciparum-infected placentas exhibited significantly higher level of MIF expression than uninfected placentas. Stimulation of syncytilized human trophoblast BeWo cells with P. falciparum-infected erythrocytes that were selected to bind these cells resulted in significant increases in MIF secretion, whereas control erythrocytes, lipopolysaccharides, and synthetic {szligbeta}-hematin had minimal effect. These findings suggest that placental malaria modulates MIF expression in different placental compartments.
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.73.6.3287-3293.2005