Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA

Listeria monocytogenes is a significant cause of food-borne disease and mortality; therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. Although conventional microarray subtyping analysis has been shown to be both high...

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2005-07, Vol.43 (7), p.3255-3259
Main Authors: Borucki, Monica K, Reynolds, James, Call, Douglas R, Ward, Todd J, Page, Brent, Kadushin, James
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
DNA
DNA - genetics
DNA Probes
food pathogens
Fundamental and applied biological sciences. Psychology
Genome, Bacterial
genotype
Infectious diseases
Listeria monocytogenes
Listeria monocytogenes - classification
Listeria monocytogenes - genetics
Medical sciences
microarray technology
Microbiology
Microspheres
Miscellaneous
new methods
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis - methods
oligonucleotide probes
pathogen identification
Polystyrenes
rapid methods
serotypes
strains
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Summary:Listeria monocytogenes is a significant cause of food-borne disease and mortality; therefore, epidemiological investigations of this pathogen require subtyping methods that are rapid, discriminatory, and reproducible. Although conventional microarray subtyping analysis has been shown to be both high resolution and genetically informative, it is still relatively low throughput and technically challenging. Suspension microarray technology eliminates the technical issues associated with planar microarrays and allows high-throughput subtyping of L. monocytogenes strains. In this study, a suspension array assay using dendrimer signal amplification allowed rapid and accurate serovar identification of L. monocytogenes strains using genomic DNA as a target. The ability to subtype genomic DNA without PCR amplification allows probes to be designed for many different regions within the bacterial genome and should allow high-resolution subtyping not possible with multiplex PCR.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.43.7.3255-3259.2005