Endothelial p21WAF1/Cip1 Cell Cycle Inhibitor is Down-regulated in Breast Cancer

Background: Tumor angiogenesis is considered a multi-pathway process, while p21 WAF1/Cip1 is a CDK inhibitor involved in cell division and survival. Herein the tumor environment effect on endothelial p21 WAF1/Cip1 expression is examined. Materials and Methods: The EA.hy 926 endothelial cell line and...

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Published in:Anticancer research 2005-07, Vol.25 (4), p.2743-2748
Main Authors: VREKOUSSIS, T, STATHOPOULOS, E. N, KAFOUSI, M, SARIDAKI, Z, SANIDAS, E, ZORAS, O
Format: Article
Language:English
Subjects:
Biological and medical sciences
Medical sciences
Tumors
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Summary:Background: Tumor angiogenesis is considered a multi-pathway process, while p21 WAF1/Cip1 is a CDK inhibitor involved in cell division and survival. Herein the tumor environment effect on endothelial p21 WAF1/Cip1 expression is examined. Materials and Methods: The EA.hy 926 endothelial cell line and tumor-conditioned medium (TCM) from the MDA-MB-468 breast cancer cell line were used. Endothelial cells grown alone and in TCM were immunostained for p21 WAF1/Cip1 and analyzed by RT-PCR. Forty-four cases of breast cancer and forty-three cases of normal breast tissue were immunostained for p21 WAF1/Cip1 . Results: Endothelial p21 WAF1/Cip1 is transcriptionally down-regulated under the influence of TCM. Moreover, it seems that breast cancer tumor endothelium does not express p21 WAF1/Cip1 . Conclusion: P21 WAF1/Cip1 plays a major role in angiogenesis, since tumor cells seem to down-regulate endothelial p21 WAF1/Cip1 , compared to endothelial cells grown in serum-free medium. The verification of the tissue culture experiment results by immunohistochemistry on tissue sections indicates p21 WAF1/Cip1 as a target of modern molecular therapy.
ISSN:0250-7005
1791-7530