Loading…
Arabidopsis RecQ14A suppresses homologous recombination and modulates DNA damage responses
The DNA damage response and DNA recombination are two interrelated mechanisms involved in maintaining the integrity of the genome, but in plants they are poorly understood. RecQ is a family of genes with conserved roles in the regulation of DNA recombination in eukaryotes; there are seven members in...
Saved in:
| Published in: | The Plant journal : for cell and molecular biology 2005, Vol.43 (6), p.789-798 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | |
| container_end_page | 798 |
| container_issue | 6 |
| container_start_page | 789 |
| container_title | The Plant journal : for cell and molecular biology |
| container_volume | 43 |
| creator | Bagherieh-Najjar, M.B Vries, O.M.H. de Hille, J Dijkwel, P.P |
| description | The DNA damage response and DNA recombination are two interrelated mechanisms involved in maintaining the integrity of the genome, but in plants they are poorly understood. RecQ is a family of genes with conserved roles in the regulation of DNA recombination in eukaryotes; there are seven members in Arabidopsis. Here we report on the functional analysis of the Arabidopsis RecQl4A gene. Ectopic expression of Arabidopsis RecQl4A in yeast RecQ-deficient cells suppressed their hypersensitivity to the DNA-damaging drug methyl methanesulfonate (MMS) and enhanced their rate of homologous recombination (HR). Analysis of three recQl4A mutant alleles revealed no obvious developmental defects or telomere deregulation in plants grown under standard growth conditions. Compared with wild-type Arabidopsis, the recQl4A mutant seedlings were found to be hypersensitive to UV light and MMS, and more resistant to mitomycin C. The average frequency of intrachromosomal HR in recQl4A mutant plants was increased 7.5-fold over that observed in wild-type plants. The data reveal roles for Arabidopsis RecQl4A in maintenance of genome stability by modulation of the DNA damage response and suppression of HR. |
| format | article |
| fullrecord | <record><control><sourceid>fao_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_17073506</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US201301031841</sourcerecordid><originalsourceid>FETCH-LOGICAL-f546-72bb091967b36de8ca4b9c75a15b00347bbb9a4b2eaa9bb3e048dbc9dc9ca07a3</originalsourceid><addsrcrecordid>eNotzM1KxDAUBeAgCo6jz2A2Lgs3TZpMlmX8hUFRRxA35d4kHSttU5KZhW9vYVwdOHznnLCFkLoqpJCfp2wBVkNhlCjP2UXOPwDCSK0W7KtOSJ2PU-4yfwvuVaia58M0pZBzyPw7DrGPu3jIPAUXB-pG3Hdx5Dh6PkR_6HE_s9vnmnsccBdmlqc4zttLdtZin8PVfy7Z9v5uu34sNi8PT-t6U7SV0oUpicAKqw1J7cPKoSLrTIWiIgCpDBHZuSsDoiWSAdTKk7PeWYdgUC7ZzfF2wuywbxOOrsvNlLoB028jDBhZgZ7d9dG1GBvcpdl8vJcgJAiQYqWE_AMc3lq_</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Arabidopsis RecQ14A suppresses homologous recombination and modulates DNA damage responses</title><source>Wiley-Blackwell Read & Publish Collection</source><source>EZB Electronic Journals Library</source><creator>Bagherieh-Najjar, M.B ; Vries, O.M.H. de ; Hille, J ; Dijkwel, P.P</creator><creatorcontrib>Bagherieh-Najjar, M.B ; Vries, O.M.H. de ; Hille, J ; Dijkwel, P.P</creatorcontrib><description>The DNA damage response and DNA recombination are two interrelated mechanisms involved in maintaining the integrity of the genome, but in plants they are poorly understood. RecQ is a family of genes with conserved roles in the regulation of DNA recombination in eukaryotes; there are seven members in Arabidopsis. Here we report on the functional analysis of the Arabidopsis RecQl4A gene. Ectopic expression of Arabidopsis RecQl4A in yeast RecQ-deficient cells suppressed their hypersensitivity to the DNA-damaging drug methyl methanesulfonate (MMS) and enhanced their rate of homologous recombination (HR). Analysis of three recQl4A mutant alleles revealed no obvious developmental defects or telomere deregulation in plants grown under standard growth conditions. Compared with wild-type Arabidopsis, the recQl4A mutant seedlings were found to be hypersensitive to UV light and MMS, and more resistant to mitomycin C. The average frequency of intrachromosomal HR in recQl4A mutant plants was increased 7.5-fold over that observed in wild-type plants. The data reveal roles for Arabidopsis RecQl4A in maintenance of genome stability by modulation of the DNA damage response and suppression of HR.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><language>eng</language><publisher>Oxford: Blackwell Science</publisher><subject>alleles ; Arabidopsis thaliana ; Biological and medical sciences ; DNA damage ; Fundamental and applied biological sciences. Psychology ; genetic recombination ; Genic rearrangement. Recombination. Transposable element ; genome instability ; genotoxicity ; hypersensitive response ; methyl methanesulfonate ; mitomycin ; Molecular and cellular biology ; Molecular genetics ; molecular sequence data ; mutants ; nucleotide sequences ; phenotypic variation ; plant genetics ; plant morphology ; plant physiology ; plant proteins ; plant response ; seedlings ; telomeres ; ultraviolet radiation</subject><ispartof>The Plant journal : for cell and molecular biology, 2005, Vol.43 (6), p.789-798</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4021</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17073506$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Bagherieh-Najjar, M.B</creatorcontrib><creatorcontrib>Vries, O.M.H. de</creatorcontrib><creatorcontrib>Hille, J</creatorcontrib><creatorcontrib>Dijkwel, P.P</creatorcontrib><title>Arabidopsis RecQ14A suppresses homologous recombination and modulates DNA damage responses</title><title>The Plant journal : for cell and molecular biology</title><description>The DNA damage response and DNA recombination are two interrelated mechanisms involved in maintaining the integrity of the genome, but in plants they are poorly understood. RecQ is a family of genes with conserved roles in the regulation of DNA recombination in eukaryotes; there are seven members in Arabidopsis. Here we report on the functional analysis of the Arabidopsis RecQl4A gene. Ectopic expression of Arabidopsis RecQl4A in yeast RecQ-deficient cells suppressed their hypersensitivity to the DNA-damaging drug methyl methanesulfonate (MMS) and enhanced their rate of homologous recombination (HR). Analysis of three recQl4A mutant alleles revealed no obvious developmental defects or telomere deregulation in plants grown under standard growth conditions. Compared with wild-type Arabidopsis, the recQl4A mutant seedlings were found to be hypersensitive to UV light and MMS, and more resistant to mitomycin C. The average frequency of intrachromosomal HR in recQl4A mutant plants was increased 7.5-fold over that observed in wild-type plants. The data reveal roles for Arabidopsis RecQl4A in maintenance of genome stability by modulation of the DNA damage response and suppression of HR.</description><subject>alleles</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>DNA damage</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic recombination</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>genome instability</subject><subject>genotoxicity</subject><subject>hypersensitive response</subject><subject>methyl methanesulfonate</subject><subject>mitomycin</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>molecular sequence data</subject><subject>mutants</subject><subject>nucleotide sequences</subject><subject>phenotypic variation</subject><subject>plant genetics</subject><subject>plant morphology</subject><subject>plant physiology</subject><subject>plant proteins</subject><subject>plant response</subject><subject>seedlings</subject><subject>telomeres</subject><subject>ultraviolet radiation</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNotzM1KxDAUBeAgCo6jz2A2Lgs3TZpMlmX8hUFRRxA35d4kHSttU5KZhW9vYVwdOHznnLCFkLoqpJCfp2wBVkNhlCjP2UXOPwDCSK0W7KtOSJ2PU-4yfwvuVaia58M0pZBzyPw7DrGPu3jIPAUXB-pG3Hdx5Dh6PkR_6HE_s9vnmnsccBdmlqc4zttLdtZin8PVfy7Z9v5uu34sNi8PT-t6U7SV0oUpicAKqw1J7cPKoSLrTIWiIgCpDBHZuSsDoiWSAdTKk7PeWYdgUC7ZzfF2wuywbxOOrsvNlLoB028jDBhZgZ7d9dG1GBvcpdl8vJcgJAiQYqWE_AMc3lq_</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Bagherieh-Najjar, M.B</creator><creator>Vries, O.M.H. de</creator><creator>Hille, J</creator><creator>Dijkwel, P.P</creator><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>2005</creationdate><title>Arabidopsis RecQ14A suppresses homologous recombination and modulates DNA damage responses</title><author>Bagherieh-Najjar, M.B ; Vries, O.M.H. de ; Hille, J ; Dijkwel, P.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f546-72bb091967b36de8ca4b9c75a15b00347bbb9a4b2eaa9bb3e048dbc9dc9ca07a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>alleles</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>DNA damage</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic recombination</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>genome instability</topic><topic>genotoxicity</topic><topic>hypersensitive response</topic><topic>methyl methanesulfonate</topic><topic>mitomycin</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>molecular sequence data</topic><topic>mutants</topic><topic>nucleotide sequences</topic><topic>phenotypic variation</topic><topic>plant genetics</topic><topic>plant morphology</topic><topic>plant physiology</topic><topic>plant proteins</topic><topic>plant response</topic><topic>seedlings</topic><topic>telomeres</topic><topic>ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bagherieh-Najjar, M.B</creatorcontrib><creatorcontrib>Vries, O.M.H. de</creatorcontrib><creatorcontrib>Hille, J</creatorcontrib><creatorcontrib>Dijkwel, P.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bagherieh-Najjar, M.B</au><au>Vries, O.M.H. de</au><au>Hille, J</au><au>Dijkwel, P.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arabidopsis RecQ14A suppresses homologous recombination and modulates DNA damage responses</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><date>2005</date><risdate>2005</risdate><volume>43</volume><issue>6</issue><spage>789</spage><epage>798</epage><pages>789-798</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>The DNA damage response and DNA recombination are two interrelated mechanisms involved in maintaining the integrity of the genome, but in plants they are poorly understood. RecQ is a family of genes with conserved roles in the regulation of DNA recombination in eukaryotes; there are seven members in Arabidopsis. Here we report on the functional analysis of the Arabidopsis RecQl4A gene. Ectopic expression of Arabidopsis RecQl4A in yeast RecQ-deficient cells suppressed their hypersensitivity to the DNA-damaging drug methyl methanesulfonate (MMS) and enhanced their rate of homologous recombination (HR). Analysis of three recQl4A mutant alleles revealed no obvious developmental defects or telomere deregulation in plants grown under standard growth conditions. Compared with wild-type Arabidopsis, the recQl4A mutant seedlings were found to be hypersensitive to UV light and MMS, and more resistant to mitomycin C. The average frequency of intrachromosomal HR in recQl4A mutant plants was increased 7.5-fold over that observed in wild-type plants. The data reveal roles for Arabidopsis RecQl4A in maintenance of genome stability by modulation of the DNA damage response and suppression of HR.</abstract><cop>Oxford</cop><pub>Blackwell Science</pub><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-7412 |
| ispartof | The Plant journal : for cell and molecular biology, 2005, Vol.43 (6), p.789-798 |
| issn | 0960-7412 1365-313X |
| language | eng |
| recordid | cdi_pascalfrancis_primary_17073506 |
| source | Wiley-Blackwell Read & Publish Collection; EZB Electronic Journals Library |
| subjects | alleles Arabidopsis thaliana Biological and medical sciences DNA damage Fundamental and applied biological sciences. Psychology genetic recombination Genic rearrangement. Recombination. Transposable element genome instability genotoxicity hypersensitive response methyl methanesulfonate mitomycin Molecular and cellular biology Molecular genetics molecular sequence data mutants nucleotide sequences phenotypic variation plant genetics plant morphology plant physiology plant proteins plant response seedlings telomeres ultraviolet radiation |
| title | Arabidopsis RecQ14A suppresses homologous recombination and modulates DNA damage responses |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T12%3A59%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Arabidopsis%20RecQ14A%20suppresses%20homologous%20recombination%20and%20modulates%20DNA%20damage%20responses&rft.jtitle=The%20Plant%20journal%20:%20for%20cell%20and%20molecular%20biology&rft.au=Bagherieh-Najjar,%20M.B&rft.date=2005&rft.volume=43&rft.issue=6&rft.spage=789&rft.epage=798&rft.pages=789-798&rft.issn=0960-7412&rft.eissn=1365-313X&rft_id=info:doi/&rft_dat=%3Cfao_pasca%3EUS201301031841%3C/fao_pasca%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-f546-72bb091967b36de8ca4b9c75a15b00347bbb9a4b2eaa9bb3e048dbc9dc9ca07a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |